Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 477038..477535 Replicon chromosome
Accession NZ_CP031916
Organism Escherichia coli O45:H2 strain FWSEC0003

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CCU02_RS02220 Protein ID WP_001173343.1
Coordinates 477362..477535 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CCU02_RS02215 Protein ID WP_000398619.1
Coordinates 477038..477334 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCU02_RS02195 473769..474488 - 720 WP_000611288.1 two-component system response regulator DcuR -
CCU02_RS02200 474485..476116 - 1632 WP_001216487.1 sensor histidine kinase -
CCU02_RS02205 476297..476527 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
CCU02_RS02210 476539..476811 + 273 WP_000405647.1 N-acetyltransferase -
CCU02_RS02215 477038..477334 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CCU02_RS02220 477362..477535 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CCU02_RS02225 477654..479171 - 1518 WP_001295074.1 lysine--tRNA ligase -
CCU02_RS02230 479408..480865 - 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T110723 WP_001173343.1 NZ_CP031916:477362-477535 [Escherichia coli O45:H2]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T110723 NZ_CP031916:477362-477535 [Escherichia coli O45:H2]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT110723 WP_000398619.1 NZ_CP031916:477038-477334 [Escherichia coli O45:H2]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT110723 NZ_CP031916:477038-477334 [Escherichia coli O45:H2]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References