Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4539257..4539875 Replicon chromosome
Accession NZ_CP031912
Organism Escherichia coli O111:NM strain FWSEC0005

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CCU03_RS22935 Protein ID WP_001291435.1
Coordinates 4539657..4539875 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CCU03_RS22930 Protein ID WP_000344800.1
Coordinates 4539257..4539631 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCU03_RS22920 4534346..4535539 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CCU03_RS22925 4535562..4538711 + 3150 WP_001132475.1 multidrug efflux RND transporter permease subunit -
CCU03_RS22930 4539257..4539631 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CCU03_RS22935 4539657..4539875 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CCU03_RS22940 4540047..4540598 + 552 WP_000102564.1 maltose O-acetyltransferase -
CCU03_RS22945 4540714..4541184 + 471 WP_001345013.1 YlaC family protein -
CCU03_RS22950 4541348..4542898 + 1551 WP_001369170.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CCU03_RS22955 4542940..4543293 - 354 WP_000878140.1 DUF1428 domain-containing protein -
CCU03_RS22965 4543672..4543983 + 312 WP_000409911.1 MGMT family protein -
CCU03_RS22970 4544014..4544586 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T110685 WP_001291435.1 NZ_CP031912:4539657-4539875 [Escherichia coli O111:NM]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T110685 NZ_CP031912:4539657-4539875 [Escherichia coli O111:NM]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT110685 WP_000344800.1 NZ_CP031912:4539257-4539631 [Escherichia coli O111:NM]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT110685 NZ_CP031912:4539257-4539631 [Escherichia coli O111:NM]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References