Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 3704263..3704734 Replicon chromosome
Accession NZ_CP031912
Organism Escherichia coli O111:NM strain FWSEC0005

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag CCU03_RS18675 Protein ID WP_001303511.1
Coordinates 3704456..3704734 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag CCU03_RS18670 Protein ID WP_001302048.1
Coordinates 3704263..3704454 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCU03_RS18635 3699591..3700121 - 531 WP_000640048.1 DUF1133 family protein -
CCU03_RS18640 3700328..3701541 + 1214 WP_162829202.1 IS3 family transposase -
CCU03_RS18645 3701601..3702023 - 423 WP_001151120.1 DUF977 family protein -
CCU03_RS18650 3702064..3703026 - 963 WP_000095671.1 helix-turn-helix domain-containing protein -
CCU03_RS18655 3703049..3703474 - 426 WP_000693943.1 Rha family transcriptional regulator -
CCU03_RS18660 3703471..3703773 - 303 WP_001444689.1 transcriptional regulator -
CCU03_RS18665 3703871..3704242 + 372 WP_001169687.1 hypothetical protein -
CCU03_RS18670 3704263..3704454 + 192 WP_001302048.1 hypothetical protein Antitoxin
CCU03_RS18675 3704456..3704734 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CCU03_RS18680 3705030..3705419 + 390 WP_001447517.1 hypothetical protein -
CCU03_RS18685 3705460..3705678 + 219 WP_001171930.1 hypothetical protein -
CCU03_RS18695 3706162..3706350 + 189 WP_000449192.1 cell division inhibition protein DicB -
CCU03_RS18700 3706347..3706538 + 192 WP_001090200.1 DUF1482 family protein -
CCU03_RS18705 3706631..3709096 + 2466 WP_000048442.1 exonuclease -
CCU03_RS18710 3709158..3709427 + 270 WP_000003742.1 excisionase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - espK / espN / espX7/nleL 3658440..3717683 59243


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T110683 WP_001303511.1 NZ_CP031912:3704456-3704734 [Escherichia coli O111:NM]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T110683 NZ_CP031912:3704456-3704734 [Escherichia coli O111:NM]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT110683 WP_001302048.1 NZ_CP031912:3704263-3704454 [Escherichia coli O111:NM]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT110683 NZ_CP031912:3704263-3704454 [Escherichia coli O111:NM]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References