Detailed information of TA system TA110671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2129276..2129901
• Replicon: chromosome
• Accession: NZ_CP031912
• Organism: Escherichia coli O111:NM strain FWSEC0005

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CCU03_RS10465
• Protein ID: WP_000911330.1
• Coordinates: 2129503..2129901 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: CCU03_RS10460
• Protein ID: WP_000450524.1
• Coordinates: 2129276..2129503 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCU03_RS10435	2125067..2125537	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
CCU03_RS10440	2125537..2126121	-	585	WP_000176194.1	glycine cleavage system transcriptional repressor	-
CCU03_RS10445	2126267..2127145	+	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CCU03_RS10450	2127162..2128196	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
CCU03_RS10455	2128409..2129122	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CCU03_RS10460	2129276..2129503	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CCU03_RS10465	2129503..2129901	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CCU03_RS10470	2130048..2130911	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
CCU03_RS10475	2130926..2132941	+	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
CCU03_RS10480	2133015..2133713	+	699	WP_000679812.1	esterase	-
CCU03_RS10485	2133823..2134023	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T110671 WP_000911330.1 NZ_CP031912:2129503-2129901 [Escherichia coli O111:NM]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T110671 NZ_CP031912:2129503-2129901 [Escherichia coli O111:NM]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT110671 WP_000450524.1 NZ_CP031912:2129276-2129503 [Escherichia coli O111:NM]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT110671 NZ_CP031912:2129276-2129503 [Escherichia coli O111:NM]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33