Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1656547..1657201 Replicon chromosome
Accession NZ_CP031912
Organism Escherichia coli O111:NM strain FWSEC0005

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag CCU03_RS08080 Protein ID WP_000244777.1
Coordinates 1656794..1657201 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CCU03_RS08075 Protein ID WP_000354046.1
Coordinates 1656547..1656813 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCU03_RS08050 1651716..1652459 + 744 WP_000951964.1 SDR family oxidoreductase -
CCU03_RS08055 1652516..1653949 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
CCU03_RS08060 1653994..1654305 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
CCU03_RS08065 1654469..1655128 + 660 WP_000250274.1 hemolysin III family protein -
CCU03_RS08070 1655324..1656304 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
CCU03_RS08075 1656547..1656813 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CCU03_RS08080 1656794..1657201 + 408 WP_000244777.1 toxin CptA Toxin
CCU03_RS08085 1657241..1657762 - 522 WP_001055874.1 flavodoxin FldB -
CCU03_RS08090 1657874..1658770 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CCU03_RS08095 1658795..1659505 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CCU03_RS08100 1659511..1661244 + 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T110668 WP_000244777.1 NZ_CP031912:1656794-1657201 [Escherichia coli O111:NM]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T110668 NZ_CP031912:1656794-1657201 [Escherichia coli O111:NM]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT110668 WP_000354046.1 NZ_CP031912:1656547-1656813 [Escherichia coli O111:NM]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT110668 NZ_CP031912:1656547-1656813 [Escherichia coli O111:NM]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References