Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 962895..963392 Replicon chromosome
Accession NZ_CP031912
Organism Escherichia coli O111:NM strain FWSEC0005

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CCU03_RS04760 Protein ID WP_001173343.1
Coordinates 963219..963392 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CCU03_RS04755 Protein ID WP_000398619.1
Coordinates 962895..963191 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCU03_RS04735 959626..960345 - 720 WP_000611288.1 two-component system response regulator DcuR -
CCU03_RS04740 960342..961973 - 1632 WP_001216487.1 sensor histidine kinase -
CCU03_RS04745 962154..962384 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
CCU03_RS04750 962396..962668 + 273 WP_000405647.1 N-acetyltransferase -
CCU03_RS04755 962895..963191 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CCU03_RS04760 963219..963392 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CCU03_RS04765 963511..964251 - 741 Protein_924 lysine--tRNA ligase -
CCU03_RS04770 964285..965498 + 1214 WP_162829202.1 IS3 family transposase -
CCU03_RS04775 965534..965695 - 162 Protein_926 IS256 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T110664 WP_001173343.1 NZ_CP031912:963219-963392 [Escherichia coli O111:NM]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T110664 NZ_CP031912:963219-963392 [Escherichia coli O111:NM]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT110664 WP_000398619.1 NZ_CP031912:962895-963191 [Escherichia coli O111:NM]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT110664 NZ_CP031912:962895-963191 [Escherichia coli O111:NM]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References