Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 4136..4779 Replicon plasmid unnamed15
Accession NZ_CP031911
Organism Escherichia coli O121:H19 strain FWSEC0006

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag B9X35_RS27435 Protein ID WP_001044768.1
Coordinates 4363..4779 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag B9X35_RS27430 Protein ID WP_001261287.1
Coordinates 4136..4366 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9X35_RS27385 1..1440 + 1440 WP_001213545.1 enterohemolysin T1SS ABC transporter subunit EhxD -
B9X35_RS27390 1507..1650 + 144 Protein_1 transcriptional regulator -
B9X35_RS27395 1654..1845 + 192 Protein_2 lipid II-degrading bacteriocin -
B9X35_RS27400 1853..2206 - 354 WP_000864810.1 colicin M immunity protein -
B9X35_RS27405 2379..3161 - 783 WP_001164205.1 site-specific integrase -
B9X35_RS27410 3163..3576 - 414 WP_000465041.1 hypothetical protein -
B9X35_RS27430 4136..4366 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
B9X35_RS27435 4363..4779 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
B9X35_RS27450 5282..6494 - 1213 Protein_8 IS3 family transposase -
B9X35_RS27455 6550..8799 - 2250 Protein_9 hypothetical protein -
B9X35_RS27460 8817..9200 - 384 WP_101329730.1 hydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyD / espP / toxB / hlyC / hlyA / hlyB 1..80681 80681


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T110662 WP_001044768.1 NZ_CP031911:4363-4779 [Escherichia coli O121:H19]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T110662 NZ_CP031911:4363-4779 [Escherichia coli O121:H19]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT110662 WP_001261287.1 NZ_CP031911:4136-4366 [Escherichia coli O121:H19]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT110662 NZ_CP031911:4136-4366 [Escherichia coli O121:H19]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References