Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 3237963..3238434 Replicon chromosome
Accession NZ_CP031910
Organism Escherichia coli O121:H19 strain FWSEC0006

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag B9X35_RS16745 Protein ID WP_001303511.1
Coordinates 3238156..3238434 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag B9X35_RS16740 Protein ID WP_001302048.1
Coordinates 3237963..3238154 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9X35_RS16700 3233012..3234568 + 1557 WP_001066422.1 IS66 family transposase -
B9X35_RS27950 3234572..3234730 - 159 WP_001370098.1 hypothetical protein -
B9X35_RS16705 3234732..3234950 - 219 WP_000209148.1 DUF4014 family protein -
B9X35_RS16710 3234983..3235195 - 213 WP_000935423.1 hypothetical protein -
B9X35_RS16715 3235301..3235723 - 423 WP_001151187.1 DUF977 family protein -
B9X35_RS16720 3235764..3236726 - 963 WP_000095671.1 helix-turn-helix domain-containing protein -
B9X35_RS16725 3236749..3237174 - 426 WP_000693944.1 Rha family transcriptional regulator -
B9X35_RS16730 3237171..3237473 - 303 WP_000172738.1 transcriptional regulator -
B9X35_RS16735 3237571..3237942 + 372 WP_001169687.1 hypothetical protein -
B9X35_RS16740 3237963..3238154 + 192 WP_001302048.1 hypothetical protein Antitoxin
B9X35_RS16745 3238156..3238434 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B9X35_RS16750 3238787..3239086 + 300 WP_001240334.1 hypothetical protein -
B9X35_RS16760 3239799..3240020 + 222 WP_000560212.1 cell division protein FtsZ -
B9X35_RS16765 3240144..3242615 + 2472 WP_000048560.1 exonuclease -
B9X35_RS16770 3242677..3242946 + 270 WP_000003742.1 excisionase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3198885..3250782 51897


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T110654 WP_001303511.1 NZ_CP031910:3238156-3238434 [Escherichia coli O121:H19]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T110654 NZ_CP031910:3238156-3238434 [Escherichia coli O121:H19]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT110654 WP_001302048.1 NZ_CP031910:3237963-3238154 [Escherichia coli O121:H19]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT110654 NZ_CP031910:3237963-3238154 [Escherichia coli O121:H19]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References