Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2699080..2699718 Replicon chromosome
Accession NZ_CP031910
Organism Escherichia coli O121:H19 strain FWSEC0006

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag B9X35_RS13545 Protein ID WP_000813794.1
Coordinates 2699542..2699718 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag B9X35_RS13540 Protein ID WP_001270286.1
Coordinates 2699080..2699496 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9X35_RS13520 2694232..2695173 - 942 WP_001251313.1 ABC transporter permease -
B9X35_RS13525 2695174..2696187 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
B9X35_RS13530 2696205..2697350 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
B9X35_RS13535 2697595..2699001 - 1407 WP_000760614.1 PLP-dependent aminotransferase family protein -
B9X35_RS13540 2699080..2699496 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
B9X35_RS13545 2699542..2699718 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
B9X35_RS13550 2699940..2700170 + 231 WP_000494244.1 YncJ family protein -
B9X35_RS13555 2700262..2702223 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
B9X35_RS13560 2702296..2702832 - 537 WP_000429141.1 DNA-binding transcriptional regulator SutR -
B9X35_RS13565 2702885..2704099 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T110653 WP_000813794.1 NZ_CP031910:c2699718-2699542 [Escherichia coli O121:H19]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T110653 NZ_CP031910:c2699718-2699542 [Escherichia coli O121:H19]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT110653 WP_001270286.1 NZ_CP031910:c2699496-2699080 [Escherichia coli O121:H19]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT110653 NZ_CP031910:c2699496-2699080 [Escherichia coli O121:H19]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCACCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References