Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 985016..985676 Replicon chromosome
Accession NZ_CP031910
Organism Escherichia coli O121:H19 strain FWSEC0006

Toxin (Protein)


Gene name cptA Uniprot ID A0A3W5XVE6
Locus tag B9X35_RS05015 Protein ID WP_000244778.1
Coordinates 985263..985676 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag B9X35_RS05010 Protein ID WP_000354046.1
Coordinates 985016..985282 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9X35_RS04985 980304..981047 + 744 WP_000951948.1 SDR family oxidoreductase -
B9X35_RS04990 981104..982537 - 1434 WP_001343615.1 6-phospho-beta-glucosidase BglA -
B9X35_RS04995 982582..982893 + 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -
B9X35_RS05000 983057..983716 + 660 WP_000250274.1 hemolysin III family protein -
B9X35_RS05005 983793..984773 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
B9X35_RS05010 985016..985282 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
B9X35_RS05015 985263..985676 + 414 WP_000244778.1 protein YgfX Toxin
B9X35_RS05020 985710..986231 - 522 WP_001055874.1 flavodoxin FldB -
B9X35_RS05025 986343..987239 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
B9X35_RS05030 987263..987973 + 711 WP_000715231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
B9X35_RS05035 987979..989712 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 16275.23 Da        Isoelectric Point: 11.7064

>T110640 WP_000244778.1 NZ_CP031910:985263-985676 [Escherichia coli O121:H19]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQRSR

Download         Length: 414 bp

>T110640 NZ_CP031910:985263-985676 [Escherichia coli O121:H19]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATCAAGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT110640 WP_000354046.1 NZ_CP031910:985016-985282 [Escherichia coli O121:H19]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT110640 NZ_CP031910:985016-985282 [Escherichia coli O121:H19]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3W5XVE6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References