Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 896708..897506 Replicon chromosome
Accession NZ_CP031910
Organism Escherichia coli O121:H19 strain FWSEC0006

Toxin (Protein)


Gene name yeeV Uniprot ID A0A3W5Y7S6
Locus tag B9X35_RS04540 Protein ID WP_000854916.1
Coordinates 896708..897085 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A3W5Y7S1
Locus tag B9X35_RS04545 Protein ID WP_024174207.1
Coordinates 897132..897506 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9X35_RS04510 892370..893083 - 714 Protein_869 DUF4942 domain-containing protein -
B9X35_RS04515 893159..893833 + 675 WP_001341423.1 IS66 family insertion sequence hypothetical protein -
B9X35_RS04520 893830..894177 + 348 WP_000631725.1 IS66 family insertion sequence element accessory protein TnpB -
B9X35_RS04525 894197..895753 + 1557 WP_001066419.1 IS66 family transposase -
B9X35_RS27860 895771..895929 - 159 WP_000014512.1 hypothetical protein -
B9X35_RS04530 896014..896211 - 198 WP_000839246.1 DUF957 domain-containing protein -
B9X35_RS04535 896223..896711 - 489 WP_000777666.1 hypothetical protein -
B9X35_RS04540 896708..897085 - 378 WP_000854916.1 TA system toxin CbtA family protein Toxin
B9X35_RS04545 897132..897506 - 375 WP_024174207.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
B9X35_RS04550 897669..897890 - 222 WP_000692312.1 DUF987 domain-containing protein -
B9X35_RS04555 897959..898093 - 135 Protein_879 hypothetical protein -
B9X35_RS04560 898134..899331 + 1198 Protein_880 IS3 family transposase -
B9X35_RS04565 899409..899759 - 351 WP_000624681.1 IS66 family insertion sequence element accessory protein TnpB -
B9X35_RS04570 899756..900181 - 426 WP_000422707.1 IS66 family insertion sequence hypothetical protein -
B9X35_RS04575 900313..900666 - 354 Protein_883 transposase -
B9X35_RS04580 900704..901170 + 467 Protein_884 transposase -
B9X35_RS04585 901175..901444 - 270 Protein_885 transposase -
B9X35_RS04590 901686..901928 + 243 WP_096855415.1 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - cesD2 / escF / escG / espF / espK 884484..913785 29301
- inside IScluster/Tn - - 890868..906198 15330


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14033.04 Da        Isoelectric Point: 9.1378

>T110639 WP_000854916.1 NZ_CP031910:c897085-896708 [Escherichia coli O121:H19]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIKAGISLCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRSNYRTVNNITPGKHPEAKQ

Download         Length: 378 bp

>T110639 NZ_CP031910:c897085-896708 [Escherichia coli O121:H19]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTACGGCCTTACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTA
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCCGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13789.59 Da        Isoelectric Point: 6.7387

>AT110639 WP_024174207.1 NZ_CP031910:c897506-897132 [Escherichia coli O121:H19]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLTIYPTPATATTS

Download         Length: 375 bp

>AT110639 NZ_CP031910:c897506-897132 [Escherichia coli O121:H19]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGACTATTTATCCGACACCGGCAACCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3W5Y7S6

Antitoxin

Source ID Structure
AlphaFold DB A0A3W5Y7S1

References