Detailed information of TA system TA110638

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 845682..846466
• Replicon: chromosome
• Accession: NZ_CP031910
• Organism: Escherichia coli O121:H19 strain FWSEC0006

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: C7BUQ8
• Locus tag: B9X35_RS04225
• Protein ID: WP_000854717.1
• Coordinates: 845682..846008 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: B9X35_RS04230
• Protein ID: WP_001285487.1
• Coordinates: 846098..846466 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9X35_RS04195	840977..841954	+	978	WP_000633206.1	type II secretion system minor pseudopilin GspK	-
B9X35_RS04200	841951..843129	+	1179	WP_000094965.1	type II secretion system protein GspL	-
B9X35_RS04205	843131..843667	+	537	WP_000942788.1	GspM family type II secretion system protein YghD	-
B9X35_RS04210	844010..844852	-	843	WP_024174205.1	DUF4942 domain-containing protein	-
B9X35_RS04215	844937..845134	-	198	WP_000839243.1	DUF957 domain-containing protein	-
B9X35_RS04220	845146..845634	-	489	WP_000761730.1	hypothetical protein	-
B9X35_RS04225	845682..846008	-	327	WP_000854717.1	TA system toxin CbtA family protein	Toxin
B9X35_RS04230	846098..846466	-	369	WP_001285487.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
B9X35_RS04235	846542..846763	-	222	WP_000692312.1	DUF987 domain-containing protein	-
B9X35_RS04240	846826..847302	-	477	WP_001186728.1	RadC family protein	-
B9X35_RS04245	847318..847791	-	474	WP_000855068.1	antirestriction protein	-
B9X35_RS04250	847885..848130	-	246	WP_032212706.1	hypothetical protein	-
B9X35_RS04255	848130..848438	-	309	Protein_819	DUF932 domain-containing protein	-
B9X35_RS04265	849720..849926	-	207	WP_000632607.1	AlpA family transcriptional regulator	-
B9X35_RS04270	850417..850590	+	174	Protein_822	N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12194.91 Da
• Isoelectric Point: 6.4697

>T110638 WP_000854717.1 NZ_CP031910:c846008-845682 [Escherichia coli O121:H19]
MKTLPDTHVREASRCPSPVIIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDN

Nucleotide

• Length: 327 bp

>T110638 NZ_CP031910:c846008-845682 [Escherichia coli O121:H19]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCATCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACTCGCGA
CAATTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13737.53 Da
• Isoelectric Point: 5.4844

>AT110638 WP_001285487.1 NZ_CP031910:c846466-846098 [Escherichia coli O121:H19]
VSDTLHETNYPNDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT110638 NZ_CP031910:c846466-846098 [Escherichia coli O121:H19]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: C7BUQ8