Detailed information of TA system TA110633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 37830..38365
• Replicon: plasmid unnamed16
• Accession: NZ_CP031909
• Organism: Escherichia coli O103:H2 strain FWSEC0007

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CCU04_RS27640
• Protein ID: WP_001270421.1
• Coordinates: 37830..38117 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: CCU04_RS27645
• Protein ID: WP_001132895.1
• Coordinates: 38114..38365 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCU04_RS27600	33233..33412	-	180	WP_000526860.1	hypothetical protein	-
CCU04_RS27605	33615..33989	+	375	WP_001368887.1	hypothetical protein	-
CCU04_RS27610	34300..34461	-	162	Protein_29	epoxyqueuosine reductase QueH	-
CCU04_RS27615	34512..34652	+	141	Protein_30	serine/threonine protein kinase	-
CCU04_RS27620	34776..35516	+	741	WP_001066926.1	tyrosine-type recombinase/integrase	-
CCU04_RS27625	35800..36777	-	978	WP_000361617.1	RepB family plasmid replication initiator protein	-
CCU04_RS27635	37423..37828	-	406	Protein_33	DDE-type integrase/transposase/recombinase	-
CCU04_RS27640	37830..38117	-	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCU04_RS27645	38114..38365	-	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CCU04_RS28080	38539..38712	-	174	WP_001435278.1	hypothetical protein	-
CCU04_RS27660	39083..40288	+	1206	WP_000817643.1	AAA family ATPase	-
CCU04_RS27665	40285..41247	+	963	WP_000756329.1	ParB/RepB/Spo0J family partition protein	-
CCU04_RS27670	41388..42550	-	1163	Protein_39	DUF4113 domain-containing protein	-
CCU04_RS27675	42431..42848	+	418	Protein_40	DUF1281 domain-containing protein	-
CCU04_RS27680	42852..43157	+	306	WP_032162675.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / stcE / exeE / exeG	1..73224	73224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T110633 WP_001270421.1 NZ_CP031909:c38117-37830 [Escherichia coli O103:H2]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T110633 NZ_CP031909:c38117-37830 [Escherichia coli O103:H2]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT110633 WP_001132895.1 NZ_CP031909:c38365-38114 [Escherichia coli O103:H2]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT110633 NZ_CP031909:c38365-38114 [Escherichia coli O103:H2]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTTTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTAGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: L4J0V6