Detailed information of TA system TA110630

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4900988..4901823
• Replicon: chromosome
• Accession: NZ_CP031908
• Organism: Escherichia coli O103:H2 strain FWSEC0007

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: F2VI91
• Locus tag: CCU04_RS25145
• Protein ID: WP_000854748.1
• Coordinates: 4901446..4901823 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0H0IVG5
• Locus tag: CCU04_RS25140
• Protein ID: WP_001339471.1
• Coordinates: 4900988..4901356 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCU04_RS25105	4897824..4898279	+	456	WP_000581504.1	hypothetical protein	-
CCU04_RS25110	4898358..4898591	+	234	WP_001119729.1	DUF905 family protein	-
CCU04_RS25120	4898691..4899509	+	819	WP_001234620.1	DUF945 domain-containing protein	-
CCU04_RS25125	4899564..4900049	+	486	WP_000849570.1	antirestriction protein	-
CCU04_RS25130	4900065..4900541	+	477	WP_001186727.1	RadC family protein	-
CCU04_RS25135	4900604..4900825	+	222	WP_000692345.1	DUF987 domain-containing protein	-
CCU04_RS25140	4900988..4901356	+	369	WP_001339471.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CCU04_RS25145	4901446..4901823	+	378	WP_000854748.1	TA system toxin CbtA family protein	Toxin
CCU04_RS25150	4901820..4902308	+	489	WP_000761721.1	hypothetical protein	-
CCU04_RS25155	4902320..4902517	+	198	WP_000839281.1	DUF957 domain-containing protein	-
CCU04_RS25160	4902614..4903186	+	573	WP_001290246.1	DUF4942 domain-containing protein	-
CCU04_RS25165	4903935..4905473	+	1539	WP_001187182.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14179.16 Da
• Isoelectric Point: 7.7761

>T110630 WP_000854748.1 NZ_CP031908:4901446-4901823 [Escherichia coli O103:H2]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYQEAKR

Nucleotide

• Length: 378 bp

>T110630 NZ_CP031908:4901446-4901823 [Escherichia coli O103:H2]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCGACTGGCCTGATGACCCGCGA
CAATTACCGAACGGTAAATAACATTACCCTGGGTAAGTATCAGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13517.22 Da
• Isoelectric Point: 6.4789

>AT110630 WP_001339471.1 NZ_CP031908:4900988-4901356 [Escherichia coli O103:H2]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT110630 NZ_CP031908:4900988-4901356 [Escherichia coli O103:H2]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: F2VI91

Antitoxin

• AlphaFold DB: A0A0H0IVG5