Detailed information of TA system TA110628

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 4672557..4673332
• Replicon: chromosome
• Accession: NZ_CP031908
• Organism: Escherichia coli O103:H2 strain FWSEC0007

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A7V7JG91
• Locus tag: CCU04_RS23960
• Protein ID: WP_001193488.1
• Coordinates: 4672557..4672928 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7V7JFU6
• Locus tag: CCU04_RS23965
• Protein ID: WP_001059301.1
• Coordinates: 4672967..4673332 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCU04_RS23930	4668515..4669231	+	717	WP_001295734.1	N-acetylneuraminic acid outer membrane channel NanC	-
CCU04_RS23935	4669251..4670357	+	1107	WP_001298927.1	N-acetylneuraminate epimerase	-
CCU04_RS23940	4670422..4671402	+	981	WP_000991444.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
CCU04_RS23960	4672557..4672928	-	372	WP_001193488.1	TA system toxin CbtA family protein	Toxin
CCU04_RS23965	4672967..4673332	-	366	WP_001059301.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CCU04_RS23970	4673358..4673579	-	222	WP_000691983.1	DUF987 domain-containing protein	-
CCU04_RS23975	4673576..4674118	-	543	WP_015740440.1	DNA repair protein RadC	-
CCU04_RS23980	4674131..4674574	-	444	WP_001096016.1	antirestriction protein	-
CCU04_RS23985	4674605..4675426	-	822	WP_001234408.1	DUF945 domain-containing protein	-
CCU04_RS23990	4675546..4676019	-	474	WP_001298943.1	hypothetical protein	-
CCU04_RS23995	4676091..4676543	-	453	WP_000734321.1	hypothetical protein	-
CCU04_RS24000	4676579..4677295	-	717	WP_000174910.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimE / fimB	4665383..4680073	14690

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13832.94 Da
• Isoelectric Point: 7.2419

>T110628 WP_001193488.1 NZ_CP031908:c4672928-4672557 [Escherichia coli O103:H2]
MQTISSHPTRATQPCLSPVEIWQRLLTCLLSQHYGLTLNDTPFSNETTILEHIDAGVSLCDAVNFLVEKYELVRIDCNDC
SVMEKSPFITSIDILRARKASGLMKRNSHKTVTRVTAGRLQEL

Nucleotide

• Length: 372 bp

>T110628 NZ_CP031908:c4672928-4672557 [Escherichia coli O103:H2]
ATGCAAACTATATCATCACACCCGACACGGGCGACTCAGCCCTGCCTGTCACCCGTTGAAATCTGGCAGCGGTTACTGAC
TTGTCTGTTGTCGCAGCACTATGGTCTGACGTTGAATGACACACCGTTCAGTAATGAAACCACCATACTGGAACACATTG
ATGCTGGAGTATCGCTGTGCGATGCGGTGAACTTTCTTGTTGAAAAATATGAACTGGTAAGAATTGACTGTAATGATTGC
TCTGTCATGGAGAAGTCACCGTTCATCACCAGTATCGATATTCTACGTGCCCGGAAAGCCTCCGGTCTGATGAAACGCAA
CAGTCACAAAACTGTTACCAGGGTAACGGCAGGTCGACTTCAGGAGCTATAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13388.25 Da
• Isoelectric Point: 5.9530

>AT110628 WP_001059301.1 NZ_CP031908:c4673332-4672967 [Escherichia coli O103:H2]
MNNHSESGTKPENPACQQWGLKCAITPCFGARLVQEGNRLHFLSDRAGFSGAFAVDVAMRLDQAFPLMMKQLELMLTSGE
LNPRHPHCVTLYHNGLTCEADTLGSCGYVYIAIYPEQTEPQ

Nucleotide

• Length: 366 bp

>AT110628 NZ_CP031908:c4673332-4672967 [Escherichia coli O103:H2]
ATGAACAATCACTCAGAATCCGGTACAAAGCCGGAGAATCCCGCCTGCCAGCAGTGGGGGCTTAAATGTGCTATTACCCC
CTGTTTTGGTGCCCGCCTGGTACAGGAGGGCAACCGATTGCATTTTCTCTCTGACCGCGCAGGATTTAGCGGGGCATTTG
CCGTCGATGTTGCAATGCGCCTTGACCAGGCATTTCCGTTAATGATGAAGCAACTTGAGCTGATGCTGACCAGTGGAGAG
CTTAACCCTCGCCACCCACACTGCGTCACGCTTTACCACAATGGACTCACCTGTGAAGCCGATACACTAGGCTCCTGTGG
TTATGTCTACATTGCTATCTATCCTGAGCAAACTGAGCCGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A7V7JG91

Antitoxin

• AlphaFold DB: A0A7V7JFU6