Detailed information of TA system TA110624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3392556..3393034
• Replicon: chromosome
• Accession: NZ_CP031908
• Organism: Escherichia coli O103:H2 strain FWSEC0007

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: CCU04_RS17680
• Protein ID: WP_001303876.1
• Coordinates: 3392747..3393034 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: CCU04_RS17675
• Protein ID: WP_000536233.1
• Coordinates: 3392556..3392747 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCU04_RS17640	3387936..3388706	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
CCU04_RS17645	3388788..3389474	-	687	WP_000788749.1	ATP-binding protein	-
CCU04_RS17650	3389481..3390596	-	1116	WP_001205820.1	hypothetical protein	-
CCU04_RS17655	3390675..3391130	+	456	WP_000273724.1	hypothetical protein	-
CCU04_RS17660	3391337..3391762	-	426	WP_000693855.1	Rha family transcriptional regulator	-
CCU04_RS17665	3391746..3392018	-	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
CCU04_RS17670	3392127..3392528	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
CCU04_RS17675	3392556..3392747	+	192	WP_000536233.1	hypothetical protein	Antitoxin
CCU04_RS17680	3392747..3393034	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCU04_RS17685	3393311..3393466	+	156	WP_000379575.1	DUF1391 family protein	-
CCU04_RS17690	3393608..3393997	+	390	WP_000394513.1	hypothetical protein	-
CCU04_RS17695	3394184..3394369	-	186	WP_001133046.1	hypothetical protein	-
CCU04_RS17705	3394943..3395131	+	189	WP_000413705.1	cell division inhibition protein DicB	-
CCU04_RS17710	3395128..3395319	+	192	WP_001098307.1	DUF1482 family protein	-
CCU04_RS17715	3395413..3397884	+	2472	WP_000034468.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3350252..3400258	50006

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T110624 WP_001303876.1 NZ_CP031908:3392747-3393034 [Escherichia coli O103:H2]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T110624 NZ_CP031908:3392747-3393034 [Escherichia coli O103:H2]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT110624 WP_000536233.1 NZ_CP031908:3392556-3392747 [Escherichia coli O103:H2]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT110624 NZ_CP031908:3392556-3392747 [Escherichia coli O103:H2]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA