Detailed information of TA system TA110611

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1481000..1481625
• Replicon: chromosome
• Accession: NZ_CP031908
• Organism: Escherichia coli O103:H2 strain FWSEC0007

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CCU04_RS07395
• Protein ID: WP_000911330.1
• Coordinates: 1481227..1481625 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: CCU04_RS07390
• Protein ID: WP_000450524.1
• Coordinates: 1481000..1481227 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCU04_RS07365	1476802..1477272	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
CCU04_RS07370	1477272..1477844	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
CCU04_RS07375	1477990..1478868	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CCU04_RS07380	1478885..1479919	+	1035	WP_001368798.1	outer membrane protein assembly factor BamC	-
CCU04_RS07385	1480132..1480845	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CCU04_RS07390	1481000..1481227	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CCU04_RS07395	1481227..1481625	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CCU04_RS07400	1481772..1482635	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
CCU04_RS07405	1482650..1484665	+	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
CCU04_RS07410	1484739..1485437	+	699	WP_000679812.1	esterase	-
CCU04_RS07415	1485547..1485747	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T110611 WP_000911330.1 NZ_CP031908:1481227-1481625 [Escherichia coli O103:H2]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T110611 NZ_CP031908:1481227-1481625 [Escherichia coli O103:H2]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT110611 WP_000450524.1 NZ_CP031908:1481000-1481227 [Escherichia coli O103:H2]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT110611 NZ_CP031908:1481000-1481227 [Escherichia coli O103:H2]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33