Detailed information of TA system TA110608

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 835707..836539
• Replicon: chromosome
• Accession: NZ_CP031908
• Organism: Escherichia coli O103:H2 strain FWSEC0007

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: CCU04_RS04140
• Protein ID: WP_000854753.1
• Coordinates: 835707..836081 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9QGA8
• Locus tag: CCU04_RS04145
• Protein ID: WP_001280948.1
• Coordinates: 836171..836539 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCU04_RS04105	830851..831489	+	639	WP_001145628.1	DUF1076 domain-containing protein	-
CCU04_RS04110	831780..833144	+	1365	WP_000953522.1	EspK/GogB family type III secretion system effector	-
CCU04_RS04125	834347..834916	-	570	WP_001290252.1	DUF4942 domain-containing protein	-
CCU04_RS04130	835013..835210	-	198	WP_000839281.1	DUF957 domain-containing protein	-
CCU04_RS04135	835222..835710	-	489	WP_000777552.1	hypothetical protein	-
CCU04_RS04140	835707..836081	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
CCU04_RS04145	836171..836539	-	369	WP_001280948.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CCU04_RS04150	836702..836923	-	222	WP_000692311.1	DUF987 domain-containing protein	-
CCU04_RS04155	836986..837462	-	477	WP_001186726.1	RadC family protein	-
CCU04_RS04160	837478..837963	-	486	WP_000849571.1	antirestriction protein	-
CCU04_RS04165	838018..838836	-	819	WP_001234620.1	DUF945 domain-containing protein	-
CCU04_RS04175	838936..839169	-	234	WP_001119729.1	DUF905 family protein	-
CCU04_RS04180	839248..839619	-	372	WP_064758448.1	hypothetical protein	-
CCU04_RS04185	839657..840870	+	1214	WP_162860179.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T110608 WP_000854753.1 NZ_CP031908:c836081-835707 [Escherichia coli O103:H2]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T110608 NZ_CP031908:c836081-835707 [Escherichia coli O103:H2]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13609.50 Da
• Isoelectric Point: 7.3991

>AT110608 WP_001280948.1 NZ_CP031908:c836539-836171 [Escherichia coli O103:H2]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT110608 NZ_CP031908:c836539-836171 [Escherichia coli O103:H2]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: A0A7U9QGA8