Detailed information of TA system TA110607

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 787954..788738
• Replicon: chromosome
• Accession: NZ_CP031908
• Organism: Escherichia coli O103:H2 strain FWSEC0007

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: C7BUQ8
• Locus tag: CCU04_RS03840
• Protein ID: WP_000854717.1
• Coordinates: 787954..788280 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: C7BUQ9
• Locus tag: CCU04_RS03845
• Protein ID: WP_001285644.1
• Coordinates: 788370..788738 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCU04_RS03810	783249..784226	+	978	WP_000633206.1	type II secretion system minor pseudopilin GspK	-
CCU04_RS03815	784223..785401	+	1179	WP_000094966.1	type II secretion system protein GspL	-
CCU04_RS03820	785403..785939	+	537	WP_000942788.1	GspM family type II secretion system protein YghD	-
CCU04_RS03825	786282..787124	-	843	WP_001378387.1	DUF4942 domain-containing protein	-
CCU04_RS03830	787209..787406	-	198	WP_000839243.1	DUF957 domain-containing protein	-
CCU04_RS03835	787418..787906	-	489	WP_000761729.1	hypothetical protein	-
CCU04_RS03840	787954..788280	-	327	WP_000854717.1	TA system toxin CbtA family protein	Toxin
CCU04_RS03845	788370..788738	-	369	WP_001285644.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CCU04_RS03850	788901..789122	-	222	WP_000692319.1	DUF987 domain-containing protein	-
CCU04_RS03855	789185..789661	-	477	WP_001368782.1	RadC family protein	-
CCU04_RS03860	789677..790150	-	474	WP_001368734.1	antirestriction protein	-
CCU04_RS03865	790244..790489	-	246	WP_001164975.1	hypothetical protein	-
CCU04_RS03870	790489..791040	-	552	Protein_753	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12194.91 Da
• Isoelectric Point: 6.4697

>T110607 WP_000854717.1 NZ_CP031908:c788280-787954 [Escherichia coli O103:H2]
MKTLPDTHVREASRCPSPVIIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDN

Nucleotide

• Length: 327 bp

>T110607 NZ_CP031908:c788280-787954 [Escherichia coli O103:H2]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCATCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACTCGCGA
CAATTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13533.31 Da
• Isoelectric Point: 5.0235

>AT110607 WP_001285644.1 NZ_CP031908:c788738-788370 [Escherichia coli O103:H2]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGVCLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT110607 NZ_CP031908:c788738-788370 [Escherichia coli O103:H2]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGTCTGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: C7BUQ8

Antitoxin

• AlphaFold DB: C7BUQ9