Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 267972..268603 Replicon chromosome
Accession NZ_CP031908
Organism Escherichia coli O103:H2 strain FWSEC0007

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag CCU04_RS01230 Protein ID WP_001259385.1
Coordinates 267972..268247 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag CCU04_RS01235 Protein ID WP_000593554.1
Coordinates 268244..268603 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCU04_RS01215 262976..264043 + 1068 WP_000361467.1 HlyD family secretion protein -
CCU04_RS01220 264040..266775 + 2736 WP_000149167.1 ribosome-associated ATPase/putative transporter RbbA -
CCU04_RS01225 266775..267899 + 1125 WP_001216257.1 ABC transporter permease -
CCU04_RS01230 267972..268247 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
CCU04_RS01235 268244..268603 + 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CCU04_RS01240 268653..269513 - 861 WP_001131783.1 class II aldolase -
CCU04_RS01245 269545..269814 - 270 WP_000933060.1 HPr family phosphocarrier protein -
CCU04_RS01250 269804..271312 - 1509 WP_001298718.1 carbohydrate kinase -
CCU04_RS01255 271305..272663 - 1359 WP_001298729.1 PTS galactitol transporter subunit IIC -
CCU04_RS01260 272740..273021 - 282 WP_000084022.1 PTS sugar transporter subunit IIB -
CCU04_RS01265 273018..273491 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 260355..268603 8248


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T110605 WP_001259385.1 NZ_CP031908:267972-268247 [Escherichia coli O103:H2]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T110605 NZ_CP031908:267972-268247 [Escherichia coli O103:H2]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT110605 WP_000593554.1 NZ_CP031908:268244-268603 [Escherichia coli O103:H2]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT110605 NZ_CP031908:268244-268603 [Escherichia coli O103:H2]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References