Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4409225..4409722 Replicon chromosome
Accession NZ_CP031906
Organism Escherichia coli O91:H21 strain FWSEC0008

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CAX06_RS21720 Protein ID WP_001173343.1
Coordinates 4409225..4409398 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CAX06_RS21725 Protein ID WP_000398619.1
Coordinates 4409426..4409722 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CAX06_RS21710 4405895..4407352 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
CAX06_RS21715 4407589..4409106 + 1518 WP_001295088.1 lysine--tRNA ligase -
CAX06_RS21720 4409225..4409398 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CAX06_RS21725 4409426..4409722 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CAX06_RS21730 4409948..4410220 - 273 WP_000405649.1 N-acetyltransferase -
CAX06_RS21735 4410232..4410462 - 231 WP_000371697.1 (4Fe-4S)-binding protein -
CAX06_RS21740 4410643..4412274 + 1632 WP_001216487.1 sensor histidine kinase -
CAX06_RS21745 4412271..4412991 + 721 Protein_4186 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T110597 WP_001173343.1 NZ_CP031906:c4409398-4409225 [Escherichia coli O91:H21]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T110597 NZ_CP031906:c4409398-4409225 [Escherichia coli O91:H21]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT110597 WP_000398619.1 NZ_CP031906:c4409722-4409426 [Escherichia coli O91:H21]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT110597 NZ_CP031906:c4409722-4409426 [Escherichia coli O91:H21]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References