Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3656780..3657398 Replicon chromosome
Accession NZ_CP031906
Organism Escherichia coli O91:H21 strain FWSEC0008

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CAX06_RS18180 Protein ID WP_001291435.1
Coordinates 3657180..3657398 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CAX06_RS18175 Protein ID WP_000344800.1
Coordinates 3656780..3657154 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CAX06_RS18165 3651869..3653062 + 1194 WP_096957999.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CAX06_RS18170 3653085..3656234 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
CAX06_RS18175 3656780..3657154 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CAX06_RS18180 3657180..3657398 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CAX06_RS18185 3657569..3658120 + 552 WP_000102564.1 maltose O-acetyltransferase -
CAX06_RS18190 3658236..3658706 + 471 WP_000136192.1 YlaC family protein -
CAX06_RS18195 3658870..3660420 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CAX06_RS18200 3660462..3660815 - 354 WP_000878141.1 DUF1428 family protein -
CAX06_RS18210 3661194..3661505 + 312 WP_000409911.1 MGMT family protein -
CAX06_RS18215 3661536..3662108 - 573 WP_000779833.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T110594 WP_001291435.1 NZ_CP031906:3657180-3657398 [Escherichia coli O91:H21]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T110594 NZ_CP031906:3657180-3657398 [Escherichia coli O91:H21]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT110594 WP_000344800.1 NZ_CP031906:3656780-3657154 [Escherichia coli O91:H21]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT110594 NZ_CP031906:3656780-3657154 [Escherichia coli O91:H21]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References