Detailed information of TA system TA110584

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1385465..1386090
• Replicon: chromosome
• Accession: NZ_CP031906
• Organism: Escherichia coli O91:H21 strain FWSEC0008

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CAX06_RS06785
• Protein ID: WP_000911330.1
• Coordinates: 1385692..1386090 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: CAX06_RS06780
• Protein ID: WP_000450524.1
• Coordinates: 1385465..1385692 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAX06_RS06755	1381268..1381738	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
CAX06_RS06760	1381738..1382310	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
CAX06_RS06765	1382456..1383334	+	879	WP_001345767.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CAX06_RS06770	1383351..1384385	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
CAX06_RS06775	1384598..1385311	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CAX06_RS06780	1385465..1385692	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CAX06_RS06785	1385692..1386090	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CAX06_RS06790	1386237..1387100	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
CAX06_RS06795	1387115..1389130	+	2016	WP_000829342.1	tRNA cytosine(34) acetyltransferase TmcA	-
CAX06_RS06800	1389204..1389902	+	699	WP_000679812.1	esterase	-
CAX06_RS06805	1389983..1390183	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T110584 WP_000911330.1 NZ_CP031906:1385692-1386090 [Escherichia coli O91:H21]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T110584 NZ_CP031906:1385692-1386090 [Escherichia coli O91:H21]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT110584 WP_000450524.1 NZ_CP031906:1385465-1385692 [Escherichia coli O91:H21]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT110584 NZ_CP031906:1385465-1385692 [Escherichia coli O91:H21]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33