Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 41411..41936 Replicon plasmid unnamed19
Accession NZ_CP031904
Organism Escherichia coli O104:H4 strain FWSEC0009

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0E0Y871
Locus tag C8200_RS27230 Protein ID WP_001159872.1
Coordinates 41631..41936 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0E0Y6P9
Locus tag C8200_RS27225 Protein ID WP_000813638.1
Coordinates 41411..41629 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C8200_RS27190 36595..36720 + 126 WP_032159539.1 hypothetical protein -
C8200_RS27195 36841..37581 + 741 WP_001066951.1 tyrosine-type recombinase/integrase -
C8200_RS27200 37860..38837 - 978 WP_001387465.1 RepB family plasmid replication initiator protein -
C8200_RS27210 39929..40078 + 150 Protein_40 transposase domain-containing protein -
C8200_RS27215 40161..40562 - 402 WP_001392130.1 type II toxin-antitoxin system VapC family toxin -
C8200_RS27220 40586..40816 - 231 WP_001261288.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
C8200_RS27225 41411..41629 + 219 WP_000813638.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C8200_RS27230 41631..41936 + 306 WP_001159872.1 type II toxin-antitoxin system toxin CcdB Toxin
C8200_RS27235 41937..42743 + 807 WP_000016961.1 site-specific integrase -
C8200_RS27240 42922..43566 + 645 WP_001144032.1 ParA family protein -
C8200_RS27245 43653..43961 + 309 WP_000030204.1 molecular chaperone GroEL -
C8200_RS27250 44375..45355 + 981 WP_000688504.1 plasmid segregation protein ParM -
C8200_RS27255 45348..45764 + 417 WP_001278815.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - aggD / aggC / aggB / aggA / aap/aspU 1..75669 75669


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11722.51 Da        Isoelectric Point: 5.6919

>T110578 WP_001159872.1 NZ_CP031904:41631-41936 [Escherichia coli O104:H4]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHWENDIKNAINLMFWGI

Download         Length: 306 bp

>T110578 NZ_CP031904:41631-41936 [Escherichia coli O104:H4]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTAATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACTGGGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8344.29 Da        Isoelectric Point: 4.5203

>AT110578 WP_000813638.1 NZ_CP031904:41411-41629 [Escherichia coli O104:H4]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVAKFIEMNGSFADENRDW

Download         Length: 219 bp

>AT110578 NZ_CP031904:41411-41629 [Escherichia coli O104:H4]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAAGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y871

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6P9

References