Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 40161..40816 Replicon plasmid unnamed19
Accession NZ_CP031904
Organism Escherichia coli O104:H4 strain FWSEC0009

Toxin (Protein)


Gene name vagD Uniprot ID A0A2H3M3N3
Locus tag C8200_RS27215 Protein ID WP_001392130.1
Coordinates 40161..40562 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A0E0YAG4
Locus tag C8200_RS27220 Protein ID WP_001261288.1
Coordinates 40586..40816 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C8200_RS27170 35299..35583 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein -
C8200_RS27175 35583..35858 + 276 WP_000421273.1 type II toxin-antitoxin system RelE/ParE family toxin -
C8200_RS27180 35864..36195 + 332 Protein_35 type II toxin-antitoxin system ParD family antitoxin -
C8200_RS27185 36311..36472 + 162 WP_001440647.1 type II toxin-antitoxin system RelE/ParE family toxin -
C8200_RS27190 36595..36720 + 126 WP_032159539.1 hypothetical protein -
C8200_RS27195 36841..37581 + 741 WP_001066951.1 tyrosine-type recombinase/integrase -
C8200_RS27200 37860..38837 - 978 WP_001387465.1 RepB family plasmid replication initiator protein -
C8200_RS27210 39929..40078 + 150 Protein_40 transposase domain-containing protein -
C8200_RS27215 40161..40562 - 402 WP_001392130.1 type II toxin-antitoxin system VapC family toxin Toxin
C8200_RS27220 40586..40816 - 231 WP_001261288.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C8200_RS27225 41411..41629 + 219 WP_000813638.1 type II toxin-antitoxin system antitoxin CcdA -
C8200_RS27230 41631..41936 + 306 WP_001159872.1 type II toxin-antitoxin system toxin CcdB -
C8200_RS27235 41937..42743 + 807 WP_000016961.1 site-specific integrase -
C8200_RS27240 42922..43566 + 645 WP_001144032.1 ParA family protein -
C8200_RS27245 43653..43961 + 309 WP_000030204.1 molecular chaperone GroEL -
C8200_RS27250 44375..45355 + 981 WP_000688504.1 plasmid segregation protein ParM -
C8200_RS27255 45348..45764 + 417 WP_001278815.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - aggD / aggC / aggB / aggA / aap/aspU 1..75669 75669


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14647.00 Da        Isoelectric Point: 6.7123

>T110577 WP_001392130.1 NZ_CP031904:c40562-40161 [Escherichia coli O104:H4]
MLDTNICSFIMREQPAAVIKRLEQAVLRNHRIVVSAITYAEMRFDATGPKASPRHIALVDAFCARLDAILPWDRAAVDAT
TEIKMALRLAETPIGPNNTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 402 bp

>T110577 NZ_CP031904:c40562-40161 [Escherichia coli O104:H4]
ATGCTCGACACGAATATCTGCTCTTTCATTATGCGTGAACAGCCCGCCGCAGTGATCAAACGTCTGGAGCAGGCGGTGCT
GCGTAACCACCGCATTGTCGTGTCGGCCATCACCTATGCGGAGATGCGTTTCGACGCCACCGGCCCGAAAGCCTCACCGC
GTCATATTGCGCTGGTTGACGCGTTCTGCGCCCGCCTCGATGCCATTCTGCCCTGGGACCGCGCTGCCGTGGACGCCACC
ACGGAGATTAAAATGGCACTGCGGCTGGCCGAGACGCCAATCGGCCCGAACAACACGGCGATTGCCGGGCACGCCATCGC
TGCCGGGGCGATACTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCTGACCTGGTGCTGGAAGACTGGGTGAAGT
AA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8684.76 Da        Isoelectric Point: 4.3654

>AT110577 WP_001261288.1 NZ_CP031904:c40816-40586 [Escherichia coli O104:H4]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWSSFAALEKADADFMAEREDIVSDEGRFDL

Download         Length: 231 bp

>AT110577 NZ_CP031904:c40816-40586 [Escherichia coli O104:H4]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCCCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTTGAGATCGTCCGCGAAGGAGACAGCATTATATTGCGCCCGGTCCGGCCGACCTGGAGCTCGTTTGCGGCACTCG
AAAAGGCCGACGCAGACTTTATGGCGGAGCGTGAGGACATTGTCAGCGACGAAGGAAGATTTGACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H3M3N3

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0YAG4

References