Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 35299..35858 | Replicon | plasmid unnamed19 |
| Accession | NZ_CP031904 | ||
| Organism | Escherichia coli O104:H4 strain FWSEC0009 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | G4VUF8 |
| Locus tag | C8200_RS27175 | Protein ID | WP_000421273.1 |
| Coordinates | 35583..35858 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | V0SRR3 |
| Locus tag | C8200_RS27170 | Protein ID | WP_001387467.1 |
| Coordinates | 35299..35583 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C8200_RS27125 | 30338..31015 | - | 678 | WP_001339397.1 | IS66 family insertion sequence hypothetical protein | - |
| C8200_RS27130 | 31080..31292 | + | 213 | Protein_27 | ANR family transcriptional regulator | - |
| C8200_RS27140 | 31743..32723 | - | 981 | WP_000019440.1 | IS5-like element ISKpn26 family transposase | - |
| C8200_RS27145 | 33004..33258 | + | 255 | WP_000083850.1 | replication regulatory protein RepA | - |
| C8200_RS27930 | 33365..33499 | + | 135 | Protein_30 | protein CopA/IncA | - |
| C8200_RS27150 | 33496..33570 | + | 75 | WP_014966221.1 | RepA leader peptide Tap | - |
| C8200_RS27155 | 33563..34391 | + | 829 | Protein_32 | incFII family plasmid replication initiator RepA | - |
| C8200_RS27170 | 35299..35583 | + | 285 | WP_001387467.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| C8200_RS27175 | 35583..35858 | + | 276 | WP_000421273.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| C8200_RS27180 | 35864..36195 | + | 332 | Protein_35 | type II toxin-antitoxin system ParD family antitoxin | - |
| C8200_RS27185 | 36311..36472 | + | 162 | WP_001440647.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| C8200_RS27190 | 36595..36720 | + | 126 | WP_032159539.1 | hypothetical protein | - |
| C8200_RS27195 | 36841..37581 | + | 741 | WP_001066951.1 | tyrosine-type recombinase/integrase | - |
| C8200_RS27200 | 37860..38837 | - | 978 | WP_001387465.1 | RepB family plasmid replication initiator protein | - |
| C8200_RS27210 | 39929..40078 | + | 150 | Protein_40 | transposase domain-containing protein | - |
| C8200_RS27215 | 40161..40562 | - | 402 | WP_001392130.1 | type II toxin-antitoxin system VapC family toxin | - |
| C8200_RS27220 | 40586..40816 | - | 231 | WP_001261288.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | aggD / aggC / aggB / aggA / aap/aspU | 1..75669 | 75669 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10765.45 Da Isoelectric Point: 9.9324
>T110576 WP_000421273.1 NZ_CP031904:35583-35858 [Escherichia coli O104:H4]
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T110576 NZ_CP031904:35583-35858 [Escherichia coli O104:H4]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10460.87 Da Isoelectric Point: 5.6558
>AT110576 WP_001387467.1 NZ_CP031904:35299-35583 [Escherichia coli O104:H4]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR
Download Length: 285 bp
>AT110576 NZ_CP031904:35299-35583 [Escherichia coli O104:H4]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0Y6H1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0Y880 |