Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4786710..4787511 Replicon chromosome
Accession NZ_CP031902
Organism Escherichia coli O104:H4 strain FWSEC0009

Toxin (Protein)


Gene name yeeV Uniprot ID D3GUT5
Locus tag C8200_RS24130 Protein ID WP_001094430.1
Coordinates 4787134..4787511 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID D3GUT4
Locus tag C8200_RS24125 Protein ID WP_001285620.1
Coordinates 4786710..4787087 (+) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C8200_RS24090 4781916..4783424 - 1509 WP_001189118.1 group II intron reverse transcriptase/maturase -
C8200_RS24100 4784097..4784836 - 740 Protein_4601 IS3 family transposase -
C8200_RS27735 4784893..4785018 + 126 Protein_4602 DUF945 domain-containing protein -
C8200_RS27740 4785018..4785191 + 174 WP_001183321.1 hypothetical protein -
C8200_RS24110 4785357..4785830 + 474 WP_001387789.1 antirestriction protein -
C8200_RS24115 4785846..4786322 + 477 WP_001390304.1 RadC family protein -
C8200_RS24120 4786409..4786630 + 222 WP_000692350.1 DUF987 domain-containing protein -
C8200_RS24125 4786710..4787087 + 378 WP_001285620.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
C8200_RS24130 4787134..4787511 + 378 WP_001094430.1 TA system toxin CbtA family protein Toxin
C8200_RS24135 4787508..4787996 + 489 WP_000761715.1 hypothetical protein -
C8200_RS24140 4788016..4788213 + 198 WP_000772029.1 DUF957 domain-containing protein -
C8200_RS24145 4788298..4789140 + 843 WP_001290187.1 DUF4942 domain-containing protein -
C8200_RS24150 4789889..4791427 + 1539 WP_001187182.1 lysine decarboxylation/transport transcriptional activator CadC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13971.82 Da        Isoelectric Point: 6.8608

>T110572 WP_001094430.1 NZ_CP031902:4787134-4787511 [Escherichia coli O104:H4]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Download         Length: 378 bp

>T110572 NZ_CP031902:4787134-4787511 [Escherichia coli O104:H4]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGAATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAAGCGAAACAATGA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13698.52 Da        Isoelectric Point: 5.9505

>AT110572 WP_001285620.1 NZ_CP031902:4786710-4787087 [Escherichia coli O104:H4]
VSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Download         Length: 378 bp

>AT110572 NZ_CP031902:4786710-4787087 [Escherichia coli O104:H4]
GTGTCAGACACACTCCCCGGGACAACGCCTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCCGACTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGTAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACTGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y6M7

Antitoxin

Source ID Structure
PDB 2INW
AlphaFold DB A0A0E0Y522

References