Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3939485..3940103 Replicon chromosome
Accession NZ_CP031902
Organism Escherichia coli O104:H4 strain FWSEC0009

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C8200_RS19955 Protein ID WP_001291435.1
Coordinates 3939885..3940103 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C8200_RS19950 Protein ID WP_000344800.1
Coordinates 3939485..3939859 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C8200_RS19940 3934574..3935767 + 1194 WP_001386618.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C8200_RS19945 3935790..3938939 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
C8200_RS19950 3939485..3939859 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C8200_RS19955 3939885..3940103 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C8200_RS19960 3940274..3940825 + 552 WP_000102564.1 maltose O-acetyltransferase -
C8200_RS19965 3940941..3941411 + 471 WP_000136192.1 YlaC family protein -
C8200_RS19970 3941575..3943125 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C8200_RS19975 3943167..3943520 - 354 WP_000878140.1 DUF1428 domain-containing protein -
C8200_RS19985 3943899..3944210 + 312 WP_000409911.1 MGMT family protein -
C8200_RS19990 3944241..3944813 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T110568 WP_001291435.1 NZ_CP031902:3939885-3940103 [Escherichia coli O104:H4]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T110568 NZ_CP031902:3939885-3940103 [Escherichia coli O104:H4]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT110568 WP_000344800.1 NZ_CP031902:3939485-3939859 [Escherichia coli O104:H4]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT110568 NZ_CP031902:3939485-3939859 [Escherichia coli O104:H4]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References