Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 860600..861401 | Replicon | chromosome |
| Accession | NZ_CP031902 | ||
| Organism | Escherichia coli O104:H4 strain FWSEC0009 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | D3GU15 |
| Locus tag | C8200_RS04320 | Protein ID | WP_001094429.1 |
| Coordinates | 860600..860977 (-) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A0E0XUQ1 |
| Locus tag | C8200_RS04325 | Protein ID | WP_001285616.1 |
| Coordinates | 861024..861401 (-) | Length | 126 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C8200_RS04285 | 855681..856280 | + | 600 | WP_001255040.1 | type II secretion system minor pseudopilin GspJ | - |
| C8200_RS04290 | 856283..857260 | + | 978 | WP_000633238.1 | type II secretion system minor pseudopilin GspK | - |
| C8200_RS04295 | 857257..858435 | + | 1179 | WP_000094962.1 | type II secretion system protein GspL | - |
| C8200_RS04300 | 858437..858973 | + | 537 | WP_000942795.1 | GspM family type II secretion system protein YghD | - |
| C8200_RS04305 | 859254..860096 | - | 843 | WP_001280493.1 | DUF4942 domain-containing protein | - |
| C8200_RS04310 | 860181..860378 | - | 198 | WP_085949090.1 | DUF957 domain-containing protein | - |
| C8200_RS04315 | 860406..860603 | - | 198 | Protein_824 | hypothetical protein | - |
| C8200_RS04320 | 860600..860977 | - | 378 | WP_001094429.1 | TA system toxin CbtA family protein | Toxin |
| C8200_RS04325 | 861024..861401 | - | 378 | WP_001285616.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| C8200_RS04330 | 861481..861702 | - | 222 | WP_000692357.1 | DUF987 domain-containing protein | - |
| C8200_RS04335 | 861789..862265 | - | 477 | WP_001388380.1 | RadC family protein | - |
| C8200_RS04340 | 862281..862754 | - | 474 | WP_000855081.1 | antirestriction protein | - |
| C8200_RS04350 | 863056..863874 | - | 819 | WP_001234603.1 | DUF945 domain-containing protein | - |
| C8200_RS04360 | 863974..864207 | - | 234 | WP_001119729.1 | DUF905 family protein | - |
| C8200_RS04365 | 864286..864741 | - | 456 | WP_000581502.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | - | gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / vipA/tssB / vipB/tssC / clbK | 764207..920723 | 156516 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 13856.73 Da Isoelectric Point: 6.8601
>T110553 WP_001094429.1 NZ_CP031902:c860977-860600 [Escherichia coli O104:H4]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ
Download Length: 378 bp
>T110553 NZ_CP031902:c860977-860600 [Escherichia coli O104:H4]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA
Antitoxin
Download Length: 126 a.a. Molecular weight: 13747.65 Da Isoelectric Point: 6.6240
>AT110553 WP_001285616.1 NZ_CP031902:c861401-861024 [Escherichia coli O104:H4]
VSDTLPGTTPPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTMTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV
VSDTLPGTTPPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTMTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV
Download Length: 378 bp
>AT110553 NZ_CP031902:c861401-861024 [Escherichia coli O104:H4]
GTGTCAGACACACTCCCCGGGACAACGCCTCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCATGACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA
GTGTCAGACACACTCCCCGGGACAACGCCTCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCATGACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0XVU5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0XUQ1 |