Detailed information of TA system TA110551

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 49335..50136
• Replicon: chromosome
• Accession: NZ_CP031902
• Organism: Escherichia coli O104:H4 strain FWSEC0009

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0E0XS49
• Locus tag: C8200_RS00250
• Protein ID: WP_001094437.1
• Coordinates: 49335..49712 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0E0XTQ1
• Locus tag: C8200_RS00255
• Protein ID: WP_001390338.1
• Coordinates: 49759..50136 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C8200_RS00220	44692..45615	-	924	WP_000535960.1	carboxylate/amino acid/amine transporter	-
C8200_RS00225	45726..46910	-	1185	WP_001172875.1	sugar efflux transporter	-
C8200_RS00235	47703..48548	-	846	WP_001274561.1	DUF4942 domain-containing protein	-
C8200_RS00240	48633..48830	-	198	WP_000772032.1	DUF957 domain-containing protein	-
C8200_RS00245	48850..49338	-	489	WP_000761716.1	hypothetical protein	-
C8200_RS00250	49335..49712	-	378	WP_001094437.1	TA system toxin CbtA family protein	Toxin
C8200_RS00255	49759..50136	-	378	WP_001390338.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C8200_RS00260	50299..50520	-	222	WP_000692300.1	DUF987 domain-containing protein	-
C8200_RS00265	50583..51059	-	477	WP_001186773.1	RadC family protein	-
C8200_RS00270	51075..51548	-	474	WP_001387789.1	antirestriction protein	-
C8200_RS00275	51714..51887	-	174	WP_001183321.1	hypothetical protein	-
C8200_RS00280	51887..52705	-	819	WP_001234716.1	DUF945 domain-containing protein	-
C8200_RS00290	52860..53018	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13986.90 Da
• Isoelectric Point: 7.3223

>T110551 WP_001094437.1 NZ_CP031902:c49712-49335 [Escherichia coli O104:H4]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T110551 NZ_CP031902:c49712-49335 [Escherichia coli O104:H4]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCATCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13745.44 Da
• Isoelectric Point: 5.0463

>AT110551 WP_001390338.1 NZ_CP031902:c50136-49759 [Escherichia coli O104:H4]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYAYLAVYPTPAEPAITV

Nucleotide

• Length: 378 bp

>AT110551 NZ_CP031902:c50136-49759 [Escherichia coli O104:H4]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAGGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGCTTATCTGGCTGTTTATCCGACACCGGCAGAGCCCGCAATCACCGTATAA

Structures

Toxin

• AlphaFold DB: A0A0E0XS49

Antitoxin

• AlphaFold DB: A0A0E0XTQ1