110550

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pasABC/RelE-HTH
Location 4134..4608 Replicon plasmid unnamed2
Accession NZ_CP031900
Organism Escherichia coli O113:H21 strain FWSEC0010

Toxin (Protein)


Gene name pasB Uniprot ID A0A748ATC0
Locus tag C8201_RS25385 Protein ID WP_000262325.1
Coordinates 4134..4400 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name pasA Uniprot ID A0A7U2VUZ4
Locus tag C8201_RS25390 Protein ID WP_000879770.1
Coordinates 4384..4608 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C8201_RS25355 806..937 - 132 WP_077521118.1 colicin release lysis protein -
C8201_RS25360 967..1257 + 291 WP_011264161.1 colicin K immunity protein -
C8201_RS25365 1254..2726 - 1473 WP_000362089.1 colicin 5 -
C8201_RS25370 2971..3261 + 291 WP_024200900.1 hypothetical protein -
C8201_RS25375 3253..3531 - 279 WP_000710675.1 hypothetical protein -
C8201_RS25380 3653..4075 + 423 WP_032319274.1 Exc2 family lipoprotein -
C8201_RS25385 4134..4400 - 267 WP_000262325.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C8201_RS25390 4384..4608 - 225 WP_000879770.1 TraY domain-containing protein Antitoxin
C8201_RS25395 4762..4974 + 213 WP_000350688.1 hypothetical protein -
C8201_RS25405 5506..5739 - 234 WP_000137429.1 MbeD family mobilization/exclusion protein -
C8201_RS25410 5746..6306 - 561 WP_001345151.1 MbeB family mobilization protein -
C8201_RS25415 6278..7021 - 744 Protein_11 relaxase/mobilization nuclease domain-containing protein -
C8201_RS25420 6984..7307 - 324 WP_001444404.1 MobC family plasmid mobilization relaxosome protein -
C8201_RS25425 7375..7566 + 192 WP_000165980.1 Rop family plasmid primer RNA-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..7769 7769


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-87)

Antitoxin

Relaxosome protein TraY

(4-45)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10455.13 Da        Isoelectric Point: 9.8720

>T110550 WP_000262325.1 NZ_CP031900:c4400-4134 [Escherichia coli O113:H21]
MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRKSGRPLKGELGEFWRYRVGDYRILCEIRDDELIILVATI
GHRREVYD

Download         Length: 267 bp

>T110550 NZ_CP031900:c4400-4134 [Escherichia coli O113:H21]
TTGGTCTGGACGATTAATTATTCCGATCGGGCGCTCAAATCGCTACGTAAGATGGACAAGCAGAACGCGCGACGGATTGT
GGATTTTATGGATTTACGTATTGCAGTTGCAGCCGATCCCCGTAAATCAGGGAGGCCACTGAAGGGGGAGTTGGGAGAAT
TCTGGCGTTATCGTGTGGGTGATTATCGCATTCTGTGTGAAATCAGGGATGACGAACTGATTATTCTTGTGGCCACCATC
GGCCATCGACGTGAAGTCTACGACTGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8369.33 Da        Isoelectric Point: 4.8552

>AT110550 WP_000879770.1 NZ_CP031900:c4608-4384 [Escherichia coli O113:H21]
MLAIRLSDEIESRLDSLAKKTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Download         Length: 225 bp

>AT110550 NZ_CP031900:c4608-4384 [Escherichia coli O113:H21]
ATGCTGGCTATTCGGTTGTCTGATGAAATTGAGTCCCGTCTGGACTCGCTGGCGAAGAAAACCGGGAGGACAAAGACGTT
CTATGCCCGGGAAGCGATACTGGCGCACCTGGAAGATCTGGAAGATTATTATCTTTCAGCGGAAACAGCAGCACGTGTTC
GCCGTGGTGATGAGGCTGTGCATTCGTCTGAGGACGTAAGGAAGTCTCTTGGTCTGGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A748ATC0

Antitoxin

Source ID Structure
AlphaFold DB A0A7U2VUZ4

References