Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 17770..18295 Replicon plasmid unnamed1
Accession NZ_CP031899
Organism Escherichia coli O113:H21 strain FWSEC0010

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag C8201_RS24525 Protein ID WP_001159871.1
Coordinates 17770..18075 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag C8201_RS24530 Protein ID WP_000813639.1
Coordinates 18077..18295 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C8201_RS24495 13765..13938 + 174 Protein_16 DUF4113 domain-containing protein -
C8201_RS24500 13940..14356 - 417 WP_001278809.1 plasmid partitioning/stability family protein -
C8201_RS24505 14349..15329 - 981 WP_000688508.1 plasmid segregation protein ParM -
C8201_RS24510 15744..16052 - 309 WP_000030199.1 hypothetical protein -
C8201_RS24515 16139..16783 - 645 WP_001144036.1 ParA family protein -
C8201_RS24520 16963..17769 - 807 WP_000016966.1 site-specific integrase -
C8201_RS24525 17770..18075 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
C8201_RS24530 18077..18295 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C8201_RS24535 18891..19151 + 261 WP_000343100.1 hypothetical protein -
C8201_RS24540 19148..19738 + 591 WP_000194569.1 hypothetical protein -
C8201_RS24545 19756..20103 - 348 WP_000142426.1 hypothetical protein -
C8201_RS24550 20573..20908 - 336 Protein_27 colicin -
C8201_RS24555 21003..21734 - 732 WP_000850413.1 complement resistance protein TraT -
C8201_RS25645 22011..22121 - 111 Protein_29 IS481 family transposase -
C8201_RS24565 22125..22336 - 212 Protein_30 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyB / hlyA / hlyC / espP 1..160712 160712


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T110549 WP_001159871.1 NZ_CP031899:c18075-17770 [Escherichia coli O113:H21]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T110549 NZ_CP031899:c18075-17770 [Escherichia coli O113:H21]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT110549 WP_000813639.1 NZ_CP031899:c18295-18077 [Escherichia coli O113:H21]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT110549 NZ_CP031899:c18295-18077 [Escherichia coli O113:H21]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGCCTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References