Detailed information of TA system TA110544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3711485..3712322
• Replicon: chromosome
• Accession: NZ_CP031898
• Organism: Escherichia coli O113:H21 strain FWSEC0010

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: C8201_RS18550
• Protein ID: WP_000227784.1
• Coordinates: 3711780..3712322 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: C8201_RS18545
• Protein ID: WP_001297137.1
• Coordinates: 3711485..3711796 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C8201_RS18520	3706505..3707452	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
C8201_RS18525	3707474..3709465	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
C8201_RS18530	3709455..3710069	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
C8201_RS18535	3710069..3710398	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
C8201_RS18540	3710410..3711300	+	891	WP_000971336.1	heme o synthase	-
C8201_RS18545	3711485..3711796	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
C8201_RS18550	3711780..3712322	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
C8201_RS18555	3712378..3713313	-	936	WP_001297127.1	sel1 repeat family protein	-
C8201_RS18560	3713721..3715085	+	1365	WP_001000978.1	MFS transporter	-
C8201_RS18565	3715213..3715704	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
C8201_RS18570	3715872..3716783	+	912	WP_000705853.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T110544 WP_000227784.1 NZ_CP031898:3711780-3712322 [Escherichia coli O113:H21]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T110544 NZ_CP031898:3711780-3712322 [Escherichia coli O113:H21]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT110544 WP_001297137.1 NZ_CP031898:3711485-3711796 [Escherichia coli O113:H21]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT110544 NZ_CP031898:3711485-3711796 [Escherichia coli O113:H21]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3