Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3677397..3678015 Replicon chromosome
Accession NZ_CP031898
Organism Escherichia coli O113:H21 strain FWSEC0010

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C8201_RS18380 Protein ID WP_001291435.1
Coordinates 3677797..3678015 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C8201_RS18375 Protein ID WP_000344800.1
Coordinates 3677397..3677771 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C8201_RS18365 3672486..3673679 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C8201_RS18370 3673702..3676851 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
C8201_RS18375 3677397..3677771 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C8201_RS18380 3677797..3678015 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C8201_RS18385 3678187..3678738 + 552 WP_000102568.1 maltose O-acetyltransferase -
C8201_RS18390 3678854..3679324 + 471 WP_000136192.1 YlaC family protein -
C8201_RS18395 3679488..3681038 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C8201_RS18400 3681080..3681433 - 354 WP_000878140.1 DUF1428 domain-containing protein -
C8201_RS18410 3681812..3682123 + 312 WP_000409911.1 MGMT family protein -
C8201_RS18415 3682154..3682726 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T110543 WP_001291435.1 NZ_CP031898:3677797-3678015 [Escherichia coli O113:H21]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T110543 NZ_CP031898:3677797-3678015 [Escherichia coli O113:H21]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT110543 WP_000344800.1 NZ_CP031898:3677397-3677771 [Escherichia coli O113:H21]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT110543 NZ_CP031898:3677397-3677771 [Escherichia coli O113:H21]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References