Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2935297..2935768 Replicon chromosome
Accession NZ_CP031898
Organism Escherichia coli O113:H21 strain FWSEC0010

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag C8201_RS14775 Protein ID WP_001303511.1
Coordinates 2935490..2935768 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag C8201_RS14770 Protein ID WP_001302048.1
Coordinates 2935297..2935488 (+) Length 64 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C8201_RS25570 2930665..2930841 - 177 WP_000753060.1 hypothetical protein -
C8201_RS14725 2930834..2931016 - 183 WP_001224672.1 hypothetical protein -
C8201_RS14730 2931164..2931469 - 306 WP_072094475.1 DUF4406 domain-containing protein -
C8201_RS14735 2931466..2931747 - 282 WP_072094476.1 DNA-binding protein -
C8201_RS14740 2931780..2932496 - 717 WP_028985606.1 DUF1627 domain-containing protein -
C8201_RS14745 2932530..2932991 - 462 WP_000139447.1 replication protein -
C8201_RS14750 2932984..2934015 - 1032 WP_050868113.1 phage replisome organizer -
C8201_RS14755 2934084..2934509 - 426 WP_000693925.1 Rha family transcriptional regulator -
C8201_RS14760 2934506..2934808 - 303 WP_032279888.1 transcriptional regulator -
C8201_RS14765 2934905..2935276 + 372 WP_001169685.1 hypothetical protein -
C8201_RS14770 2935297..2935488 + 192 WP_001302048.1 hypothetical protein Antitoxin
C8201_RS14775 2935490..2935768 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C8201_RS14780 2936059..2936211 + 153 WP_000367379.1 DUF1391 family protein -
C8201_RS14785 2936325..2936840 - 516 WP_000559920.1 hypothetical protein -
C8201_RS14795 2937369..2937557 + 189 WP_000450222.1 cell division inhibitor -
C8201_RS14800 2937554..2937742 + 189 WP_000092784.1 DUF1482 family protein -
C8201_RS14805 2937835..2940279 + 2445 WP_000102189.1 exonuclease -
C8201_RS14810 2940341..2940610 + 270 WP_000003742.1 excisionase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - stx2B / stx2A 2915147..2945005 29858


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-91)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T110542 WP_001303511.1 NZ_CP031898:2935490-2935768 [Escherichia coli O113:H21]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T110542 NZ_CP031898:2935490-2935768 [Escherichia coli O113:H21]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCAGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTGGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT110542 WP_001302048.1 NZ_CP031898:2935297-2935488 [Escherichia coli O113:H21]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT110542 NZ_CP031898:2935297-2935488 [Escherichia coli O113:H21]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAGTTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAAATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE


Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References