Detailed information of TA system TA110531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1382686..1383311
• Replicon: chromosome
• Accession: NZ_CP031898
• Organism: Escherichia coli O113:H21 strain FWSEC0010

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C8201_RS06775
• Protein ID: WP_000911330.1
• Coordinates: 1382913..1383311 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C8201_RS06770
• Protein ID: WP_000450524.1
• Coordinates: 1382686..1382913 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C8201_RS06745	1378489..1378959	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
C8201_RS06750	1378959..1379531	-	573	WP_000176191.1	glycine cleavage system transcriptional repressor	-
C8201_RS06755	1379677..1380555	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C8201_RS06760	1380572..1381606	+	1035	WP_001358397.1	outer membrane protein assembly factor BamC	-
C8201_RS06765	1381819..1382532	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C8201_RS06770	1382686..1382913	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C8201_RS06775	1382913..1383311	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C8201_RS06780	1383458..1384321	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
C8201_RS06785	1384336..1386351	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
C8201_RS06790	1386425..1387123	+	699	WP_000679807.1	esterase	-
C8201_RS06795	1387233..1387433	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T110531 WP_000911330.1 NZ_CP031898:1382913-1383311 [Escherichia coli O113:H21]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T110531 NZ_CP031898:1382913-1383311 [Escherichia coli O113:H21]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT110531 WP_000450524.1 NZ_CP031898:1382686-1382913 [Escherichia coli O113:H21]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT110531 NZ_CP031898:1382686-1382913 [Escherichia coli O113:H21]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33