Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5161971..5162596 Replicon chromosome
Accession NZ_CP031885
Organism Klebsiella pneumoniae strain WCHKP095845

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag BT110_RS27260 Protein ID WP_019705794.1
Coordinates 5161971..5162354 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag BT110_RS27265 Protein ID WP_004150355.1
Coordinates 5162354..5162596 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BT110_RS27245 5159337..5160239 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
BT110_RS27250 5160236..5160871 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
BT110_RS27255 5160868..5161797 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
BT110_RS27260 5161971..5162354 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
BT110_RS27265 5162354..5162596 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
BT110_RS27270 5162801..5163718 + 918 WP_004146235.1 alpha/beta hydrolase -
BT110_RS27275 5163733..5164674 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
BT110_RS27280 5164719..5165156 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
BT110_RS27285 5165153..5166013 - 861 WP_004146232.1 virulence factor BrkB family protein -
BT110_RS27290 5166007..5166606 - 600 WP_072310962.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T110484 WP_019705794.1 NZ_CP031885:c5162354-5161971 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T110484 NZ_CP031885:c5162354-5161971 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCATCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT110484 WP_004150355.1 NZ_CP031885:c5162596-5162354 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT110484 NZ_CP031885:c5162596-5162354 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References