Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 67888..68413 Replicon plasmid pMCR8_095845
Accession NZ_CP031883
Organism Klebsiella pneumoniae strain WCHKP095845

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag BT110_RS00510 Protein ID WP_013023785.1
Coordinates 68108..68413 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag BT110_RS00505 Protein ID WP_001568025.1
Coordinates 67888..68106 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BT110_RS00470 63044..63823 + 780 WP_013023780.1 hypothetical protein -
BT110_RS00475 64030..65622 + 1593 WP_015344964.1 hypothetical protein -
BT110_RS00480 65656..66783 + 1128 WP_013023782.1 DUF4238 domain-containing protein -
BT110_RS00485 66780..67070 + 291 WP_013023783.1 hypothetical protein -
BT110_RS00505 67888..68106 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
BT110_RS00510 68108..68413 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
BT110_RS00515 68582..68977 + 396 WP_017899885.1 hypothetical protein -
BT110_RS00520 69004..69318 + 315 WP_053389906.1 hypothetical protein -
BT110_RS00525 69329..70345 + 1017 WP_017899884.1 hypothetical protein -
BT110_RS00530 70543..71337 + 795 WP_004197635.1 site-specific integrase -
BT110_RS00535 71801..71980 - 180 WP_006788217.1 hypothetical protein -
BT110_RS00540 72100..72726 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-8 / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1A - 1..110966 110966


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T110467 WP_013023785.1 NZ_CP031883:68108-68413 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T110467 NZ_CP031883:68108-68413 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT110467 WP_001568025.1 NZ_CP031883:67888-68106 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT110467 NZ_CP031883:67888-68106 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References