Detailed information of TA system TA110466

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 2056651..2057366
• Replicon: chromosome
• Accession: NZ_CP031875
• Organism: Actinobacillus pleuropneumoniae serovar

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D1095_RS09675
• Protein ID: WP_005599445.1
• Coordinates: 2056651..2057016 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BT01
• Locus tag: D1095_RS09680
• Protein ID: WP_005599447.1
• Coordinates: 2057079..2057366 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1095_RS09660 (D1095_09750)	2052236..2053942	-	1707	WP_005602678.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-
D1095_RS09665 (D1095_09755)	2054038..2054319	-	282	WP_005599441.1	DNA-directed RNA polymerase subunit omega	-
D1095_RS09670 (D1095_09760)	2054446..2056527	-	2082	WP_005602680.1	ATP-dependent DNA helicase RecG	-
D1095_RS09675 (D1095_09765)	2056651..2057016	-	366	WP_005599445.1	type II toxin-antitoxin system VapC family toxin	Toxin
D1095_RS09680 (D1095_09770)	2057079..2057366	-	288	WP_005599447.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
D1095_RS09685 (D1095_09775)	2057572..2059287	+	1716	WP_005602682.1	proline--tRNA ligase	-
D1095_RS09690 (D1095_09780)	2059353..2059541	-	189	WP_005602684.1	hypothetical protein	-
D1095_RS09695 (D1095_09785)	2059544..2060125	-	582	WP_005599450.1	sigma-70 family RNA polymerase sigma factor	-
D1095_RS09700 (D1095_09790)	2060214..2061170	+	957	WP_005619040.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
D1095_RS09705 (D1095_09795)	2061170..2061763	+	594	WP_005599453.1	protein-methionine-sulfoxide reductase heme-binding subunit MsrQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13974.06 Da
• Isoelectric Point: 9.0549

>T110466 WP_005599445.1 NZ_CP031875:c2057016-2056651 [Actinobacillus pleuropneumoniae serovar 2 str. S1536]
MDTNVRQWLATIKPSQTNISCITLSEIKTGILLKARKDPIQAERLNHWFTHNVLSVYQAKSFSINNEIALLASEYHIPNK
MDLNDAYIAATAKYHNLVLVTRNLKDFNRCDIRLFNPFEPN

Nucleotide

• Length: 366 bp

>T110466 NZ_CP031875:c2057016-2056651 [Actinobacillus pleuropneumoniae serovar 2 str. S1536]
ATGGATACCAATGTACGACAATGGTTAGCAACGATTAAACCGAGTCAGACGAATATTAGCTGTATCACGCTTTCCGAAAT
AAAAACGGGGATTTTACTCAAAGCTCGTAAAGATCCTATTCAAGCCGAGCGGTTAAATCATTGGTTTACCCATAATGTAT
TAAGCGTTTATCAAGCTAAATCCTTTTCTATAAATAATGAAATTGCGTTACTGGCTTCTGAATACCACATTCCAAATAAA
ATGGATTTAAACGATGCTTATATTGCCGCAACCGCTAAATATCATAATTTAGTGCTTGTTACTCGTAATTTGAAAGACTT
CAACCGTTGCGATATACGTTTATTTAATCCTTTCGAACCGAATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11040.29 Da
• Isoelectric Point: 4.5578

>AT110466 WP_005599447.1 NZ_CP031875:c2057366-2057079 [Actinobacillus pleuropneumoniae serovar 2 str. S1536]
MPITITSREFNQHASKVLKMAEKEPVFITKWGKIVSVVSNYVDYKQHTTVEPSFEELFASSTDESVSDEFWQDFEQTLTK
IRKSNKSEQIDFGDE

Nucleotide

• Length: 288 bp

>AT110466 NZ_CP031875:c2057366-2057079 [Actinobacillus pleuropneumoniae serovar 2 str. S1536]
ATGCCAATTACTATTACCTCTCGAGAATTTAATCAACACGCCAGTAAAGTGCTGAAAATGGCAGAAAAAGAACCGGTTTT
TATCACTAAATGGGGAAAAATTGTCAGCGTTGTCTCCAATTATGTTGATTACAAACAGCACACAACGGTTGAGCCTAGCT
TTGAAGAACTATTTGCCTCATCTACGGATGAATCGGTTAGCGATGAGTTTTGGCAAGATTTTGAACAGACATTAACAAAA
ATTCGCAAAAGTAATAAATCCGAACAAATAGATTTTGGAGATGAATAA

Structures

Antitoxin

• AlphaFold DB: A3N3C5