Detailed information of TA system TA110465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1959527..1960155
• Replicon: chromosome
• Accession: NZ_CP031875
• Organism: Actinobacillus pleuropneumoniae serovar

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D1095_RS09095
• Protein ID: WP_005602539.1
• Coordinates: 1959757..1960155 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A3S5F5Z7
• Locus tag: D1095_RS09090
• Protein ID: WP_005602537.1
• Coordinates: 1959527..1959757 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1095_RS09075 (D1095_09170)	1956834..1958384	+	1551	WP_005602533.1	class I SAM-dependent methyltransferase	-
D1095_RS09080 (D1095_09175)	1958381..1959163	+	783	WP_005602534.1	HincII family type II restriction endonuclease	-
D1095_RS09085	1959279..1959419	-	141	WP_005602535.1	hypothetical protein	-
D1095_RS09090 (D1095_09180)	1959527..1959757	+	231	WP_005602537.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D1095_RS09095 (D1095_09185)	1959757..1960155	+	399	WP_005602539.1	tRNA(fMet)-specific endonuclease VapC	Toxin
D1095_RS09100 (D1095_09190)	1960291..1960707	-	417	WP_005602541.1	DUF417 family protein	-
D1095_RS09105 (D1095_09195)	1960953..1961603	+	651	WP_005602543.1	DUF2202 domain-containing protein	-
D1095_RS09110 (D1095_09200)	1961783..1962562	-	780	WP_005602546.1	hypothetical protein	-
D1095_RS09115 (D1095_09205)	1962635..1963957	-	1323	WP_005620148.1	HslU--HslV peptidase ATPase subunit	-
D1095_RS09120 (D1095_09210)	1964103..1964624	-	522	WP_005613306.1	ATP-dependent protease subunit HslV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1944489..2001101	56612

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (6-53)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15003.30 Da
• Isoelectric Point: 8.0810

>T110465 WP_005602539.1 NZ_CP031875:1959757-1960155 [Actinobacillus pleuropneumoniae serovar 2 str. S1536]
MLAYMLDTNIAIYVIKRRPIEVLDKFNLNSTRLCVSSITAAELYYGAEKSQFPERNMAVIEDFLSRLTILDYTHKAATHF
GNIKAHLSKQGKIIGENDIHIAAHARSEGLVLVTNNLRAFERVEGLRLDNWV

Nucleotide

• Length: 399 bp

>T110465 NZ_CP031875:1959757-1960155 [Actinobacillus pleuropneumoniae serovar 2 str. S1536]
ATGCTGGCTTATATGCTTGATACTAATATTGCGATTTATGTGATTAAACGCAGACCTATTGAAGTGCTGGATAAGTTTAA
TTTAAATTCTACTCGTCTTTGTGTAAGTAGTATCACTGCAGCGGAGTTGTATTATGGTGCAGAGAAAAGCCAGTTTCCGG
AACGAAATATGGCAGTAATTGAGGATTTTCTTTCTCGTTTAACGATTCTTGATTATACGCATAAGGCGGCTACTCACTTT
GGCAATATTAAAGCACATTTATCTAAGCAAGGTAAGATCATTGGCGAGAACGATATTCATATTGCCGCACATGCAAGAAG
TGAAGGACTCGTATTAGTGACCAATAACTTGCGTGCATTTGAACGTGTGGAAGGATTGCGATTAGATAATTGGGTATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8591.74 Da
• Isoelectric Point: 4.7063

>AT110465 WP_005602537.1 NZ_CP031875:1959527-1959757 [Actinobacillus pleuropneumoniae serovar 2 str. S1536]
MEASVFMTNRSQAVRLPAEVRFASDVKKLSVRVVGNERILAPINQSWDSFFLSSATVSDDFMTEREISIQPEREGL

Nucleotide

• Length: 231 bp

>AT110465 NZ_CP031875:1959527-1959757 [Actinobacillus pleuropneumoniae serovar 2 str. S1536]
ATGGAAGCATCGGTATTTATGACAAACCGTAGTCAGGCGGTACGATTACCTGCAGAAGTGCGTTTTGCAAGTGATGTAAA
AAAACTTTCTGTAAGAGTGGTAGGCAATGAACGTATTTTGGCTCCAATTAACCAAAGTTGGGATAGCTTCTTTCTGAGTT
CGGCAACGGTAAGCGATGATTTCATGACAGAGCGTGAAATCAGCATTCAGCCCGAACGTGAGGGACTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3S5F5Z7