Detailed information of TA system TA110462

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 1442893..1443401
• Replicon: chromosome
• Accession: NZ_CP031875
• Organism: Actinobacillus pleuropneumoniae serovar

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B0BQK9
• Locus tag: D1095_RS06525
• Protein ID: WP_005605064.1
• Coordinates: 1443141..1443401 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B0BQK8
• Locus tag: D1095_RS06520
• Protein ID: WP_005598318.1
• Coordinates: 1442893..1443144 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1095_RS06485 (D1095_06575)	1438555..1439103	-	549	WP_005601803.1	type IV pilus biogenesis/stability protein PilW	-
D1095_RS06490 (D1095_06580)	1439157..1440338	-	1182	WP_005601805.1	bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN	-
D1095_RS06510 (D1095_06600)	1441116..1442555	+	1440	WP_005601815.1	glutamate--tRNA ligase	-
D1095_RS06520 (D1095_06610)	1442893..1443144	+	252	WP_005598318.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
D1095_RS06525 (D1095_06615)	1443141..1443401	+	261	WP_005605064.1	Txe/YoeB family addiction module toxin	Toxin
D1095_RS06530 (D1095_06620)	1443560..1443727	-	168	WP_005598320.1	Trm112 family protein	-
D1095_RS06535 (D1095_06625)	1443729..1444709	-	981	WP_005601819.1	tetraacyldisaccharide 4'-kinase	-
D1095_RS06540 (D1095_06630)	1444803..1446062	-	1260	WP_005598324.1	ATP-dependent protease ATP-binding subunit ClpX	-
D1095_RS06545 (D1095_06635)	1446062..1446652	-	591	WP_005601821.1	ATP-dependent Clp endopeptidase proteolytic subunit ClpP	-
D1095_RS06550 (D1095_06640)	1446776..1447168	+	393	WP_005618838.1	SufE family protein	-
D1095_RS06565 (D1095_06655)	1447527..1448300	-	774	WP_005618837.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10423.99 Da
• Isoelectric Point: 8.0109

>T110462 WP_005605064.1 NZ_CP031875:1443141-1443401 [Actinobacillus pleuropneumoniae serovar 2 str. S1536]
MKLTFSSNAWEDYLYWQKTDKIILKRINSLIKDIQRQPFEGIGKLEPLKFNLSGFWSRRINEEHRLIYSVEDEAILIVAC
RYHYDQ

Nucleotide

• Length: 261 bp

>T110462 NZ_CP031875:1443141-1443401 [Actinobacillus pleuropneumoniae serovar 2 str. S1536]
ATGAAGCTAACTTTCTCGTCTAATGCGTGGGAAGATTATTTATACTGGCAAAAAACCGATAAAATAATTTTGAAACGTAT
AAACAGCCTGATCAAAGATATTCAACGCCAACCATTCGAAGGCATAGGAAAACTGGAGCCGCTAAAATTCAATCTTTCGG
GATTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACAGCGTGGAAGATGAAGCAATTTTAATAGTAGCGTGT
AGATACCATTATGATCAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9362.66 Da
• Isoelectric Point: 4.5659

>AT110462 WP_005598318.1 NZ_CP031875:1442893-1443144 [Actinobacillus pleuropneumoniae serovar 2 str. S1536]
MQAITYTEARQNLAGTMSKVAEDFEPILITRSKGGNCVLMSYEQYCSLEETAYLMRSPTNAKRLLDSVAELRQGQGIERE
LIE

Nucleotide

• Length: 252 bp

>AT110462 NZ_CP031875:1442893-1443144 [Actinobacillus pleuropneumoniae serovar 2 str. S1536]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATCTAGCCGGTACGATGTCAAAAGTAGCAGAAGATTTTGAGCCTAT
TTTAATTACAAGAAGCAAGGGCGGAAATTGCGTACTTATGTCGTATGAGCAGTATTGTTCACTTGAAGAAACCGCCTATT
TGATGCGTTCACCCACCAATGCAAAACGGCTATTGGATAGTGTGGCGGAATTAAGACAGGGACAAGGCATTGAAAGAGAG
CTAATCGAATGA

Structures

Toxin

• AlphaFold DB: B0BQK9

Antitoxin

• AlphaFold DB: B0BQK8