Detailed information of TA system TA110461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 2006843..2007558
• Replicon: chromosome
• Accession: NZ_CP031874
• Organism: Actinobacillus pleuropneumoniae strain S1421

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D1096_RS09350
• Protein ID: WP_052290829.1
• Coordinates: 2006843..2007208 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BT01
• Locus tag: D1096_RS09355
• Protein ID: WP_005599447.1
• Coordinates: 2007271..2007558 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1096_RS09335 (D1096_09415)	2002428..2004134	-	1707	WP_005606484.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-
D1096_RS09340 (D1096_09420)	2004230..2004511	-	282	WP_012263402.1	DNA-directed RNA polymerase subunit omega	-
D1096_RS09345 (D1096_09425)	2004638..2006719	-	2082	WP_012263403.1	ATP-dependent DNA helicase RecG	-
D1096_RS09350 (D1096_09430)	2006843..2007208	-	366	WP_052290829.1	type II toxin-antitoxin system VapC family toxin	Toxin
D1096_RS09355 (D1096_09435)	2007271..2007558	-	288	WP_005599447.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
D1096_RS09360 (D1096_09440)	2007764..2009479	+	1716	WP_012263406.1	proline--tRNA ligase	-
D1096_RS09365 (D1096_09445)	2009545..2009733	-	189	WP_005602684.1	hypothetical protein	-
D1096_RS09370 (D1096_09450)	2009736..2010317	-	582	WP_005606496.1	sigma-70 family RNA polymerase sigma factor	-
D1096_RS09375 (D1096_09455)	2010406..2011362	+	957	WP_012263408.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
D1096_RS09380 (D1096_09460)	2011362..2011955	+	594	WP_005599453.1	protein-methionine-sulfoxide reductase heme-binding subunit MsrQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13961.01 Da
• Isoelectric Point: 7.7998

>T110461 WP_052290829.1 NZ_CP031874:c2007208-2006843 [Actinobacillus pleuropneumoniae]
IDTNVRQWLETIKPSQTNISCITLSEIKTGILLKARKDPIQAERLNHWFTHNVLSVYQAKSFSINNEIALLASEYHIPNK
MDLNDAYIAATAKYHNLVLVTRNLKDFNCCDIRLFNPFEPN

Nucleotide

• Length: 366 bp

>T110461 NZ_CP031874:c2007208-2006843 [Actinobacillus pleuropneumoniae]
ATAGATACCAATGTACGACAATGGTTAGAAACGATTAAACCGAGTCAGACGAATATTAGCTGTATCACGCTTTCCGAAAT
AAAAACGGGGATTTTACTTAAAGCTCGTAAAGATCCTATTCAAGCCGAGCGGTTAAATCATTGGTTTACCCATAATGTAT
TAAGCGTTTATCAAGCTAAATCCTTTTCTATAAATAATGAAATTGCGTTACTGGCTTCTGAATACCACATTCCAAATAAA
ATGGATTTAAACGATGCTTATATTGCCGCAACCGCTAAATATCATAATTTAGTGCTTGTTACTCGTAATTTGAAAGACTT
CAACTGTTGCGATATACGTTTATTTAATCCTTTCGAACCGAATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11040.29 Da
• Isoelectric Point: 4.5578

>AT110461 WP_005599447.1 NZ_CP031874:c2007558-2007271 [Actinobacillus pleuropneumoniae]
MPITITSREFNQHASKVLKMAEKEPVFITKWGKIVSVVSNYVDYKQHTTVEPSFEELFASSTDESVSDEFWQDFEQTLTK
IRKSNKSEQIDFGDE

Nucleotide

• Length: 288 bp

>AT110461 NZ_CP031874:c2007558-2007271 [Actinobacillus pleuropneumoniae]
ATGCCAATTACTATTACCTCTCGAGAATTTAATCAACACGCCAGTAAAGTGCTGAAAATGGCAGAAAAAGAACCGGTTTT
TATCACTAAATGGGGAAAAATTGTCAGCGTTGTCTCCAATTATGTTGATTACAAACAGCACACAACGGTTGAGCCTAGCT
TTGAAGAACTATTTGCCTCATCTACGGATGAATCGGTTAGCGATGAGTTTTGGCAAGATTTTGAACAGACATTAACAAAA
ATTCGCAAAAGTAATAAATCCGAACAAATAGATTTTGGAGATGAATAA

Structures

Antitoxin

• AlphaFold DB: A3N3C5