Detailed information of TA system TA110459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1706964..1707695
• Replicon: chromosome
• Accession: NZ_CP031874
• Organism: Actinobacillus pleuropneumoniae strain S1421

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: B0BRD2
• Locus tag: D1096_RS07785
• Protein ID: WP_005605461.1
• Coordinates: 1706964..1707431 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: B0BRD3
• Locus tag: D1096_RS07790
• Protein ID: WP_005619269.1
• Coordinates: 1707435..1707695 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1096_RS07760 (D1096_07815)	1702319..1702690	-	372	WP_005598830.1	CrcB family protein	-
D1096_RS07765 (D1096_07820)	1702693..1703757	-	1065	WP_011848610.1	MFS transporter	-
D1096_RS07770 (D1096_07825)	1703852..1704961	+	1110	WP_012263272.1	anhydro-N-acetylmuramic acid kinase	-
D1096_RS07775 (D1096_07830)	1705015..1705929	+	915	WP_012263273.1	N-acetylmuramic acid 6-phosphate etherase	-
D1096_RS07780 (D1096_07835)	1705999..1706880	-	882	WP_012263274.1	50S ribosomal protein L11 methyltransferase	-
D1096_RS07785 (D1096_07840)	1706964..1707431	-	468	WP_005605461.1	GNAT family N-acetyltransferase	Toxin
D1096_RS07790 (D1096_07845)	1707435..1707695	-	261	WP_005619269.1	DUF1778 domain-containing protein	Antitoxin
D1096_RS07795 (D1096_07850)	1707816..1709276	-	1461	WP_012263275.1	metalloprotease TldD	-
D1096_RS07800 (D1096_07855)	1709406..1710665	+	1260	WP_005618010.1	tRNA lysidine(34) synthetase TilS	-
D1096_RS07805 (D1096_07860)	1710692..1710982	-	291	WP_005602215.1	DUF5389 family protein	-
D1096_RS07810 (D1096_07865)	1710984..1711877	-	894	WP_005608892.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (40-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17276.25 Da
• Isoelectric Point: 9.5125

>T110459 WP_005605461.1 NZ_CP031874:c1707431-1706964 [Actinobacillus pleuropneumoniae]
MHAPELLSEQHIVRYFQCGEVVLDQWLQRTALKNQQNNASKTFVVRDENKQVMGFYCLSAGSVSHQFVAGALRRNMPDPI
PVIILGRLAVDCSAQGKQLGVSMLKDAVLKSKTVANQIGVKALLVHALNQQAKQFYLKYGFSCSPIDEMVLMLKL

Nucleotide

• Length: 468 bp

>T110459 NZ_CP031874:c1707431-1706964 [Actinobacillus pleuropneumoniae]
ATGCACGCACCGGAACTGCTTTCCGAGCAACATATTGTGCGATATTTTCAATGTGGTGAGGTCGTGCTAGACCAATGGCT
ACAACGTACTGCATTGAAAAATCAGCAAAATAATGCCAGTAAAACCTTTGTTGTGCGTGATGAGAATAAACAGGTGATGG
GATTTTATTGTTTATCTGCCGGTTCGGTGAGCCATCAGTTTGTTGCAGGCGCATTAAGACGTAATATGCCCGATCCGATT
CCCGTGATTATTTTAGGTAGATTAGCGGTCGATTGTTCGGCACAGGGTAAGCAGTTAGGCGTATCAATGTTAAAAGATGC
GGTCTTAAAATCGAAGACGGTAGCGAATCAAATCGGGGTAAAAGCATTGCTTGTTCATGCGTTAAATCAGCAAGCAAAAC
AATTTTATTTAAAGTACGGTTTTTCATGTTCACCCATTGATGAGATGGTTTTAATGTTAAAACTTTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9796.14 Da
• Isoelectric Point: 4.4883

>AT110459 WP_005619269.1 NZ_CP031874:c1707695-1707435 [Actinobacillus pleuropneumoniae]
MRTAPINLRAMPDQRDLIDYAASILGKTRSDFMLETACQAAQNVILDRTVFQLNEQAFEQFNRLLEQPTADNPGLDKLLN
TKAVWE

Nucleotide

• Length: 261 bp

>AT110459 NZ_CP031874:c1707695-1707435 [Actinobacillus pleuropneumoniae]
ATGAGAACTGCGCCAATTAATTTACGAGCGATGCCTGATCAGCGTGATTTAATTGATTATGCGGCAAGCATTTTAGGTAA
AACACGTTCTGACTTTATGTTAGAAACCGCCTGCCAAGCTGCCCAAAATGTGATTTTAGATCGTACCGTTTTCCAATTAA
ATGAGCAGGCTTTCGAGCAATTTAACCGATTGCTAGAACAGCCAACAGCAGATAATCCGGGGTTAGATAAACTGTTAAAT
ACCAAAGCGGTGTGGGAGTAG

Structures

Toxin

• AlphaFold DB: B0BRD2

Antitoxin

• AlphaFold DB: A3N2I7