Detailed information of TA system TA110457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 1411099..1411607
• Replicon: chromosome
• Accession: NZ_CP031874
• Organism: Actinobacillus pleuropneumoniae strain S1421

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B0BQK9
• Locus tag: D1096_RS06320
• Protein ID: WP_005605064.1
• Coordinates: 1411347..1411607 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B0BQK8
• Locus tag: D1096_RS06315
• Protein ID: WP_005598318.1
• Coordinates: 1411099..1411350 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1096_RS06275 (D1096_06330)	1406634..1407182	-	549	WP_012263163.1	type IV pilus biogenesis/stability protein PilW	-
D1096_RS06280 (D1096_06335)	1407236..1408417	-	1182	WP_005608495.1	bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN	-
D1096_RS06305 (D1096_06360)	1409322..1410761	+	1440	WP_005605060.1	glutamate--tRNA ligase	-
D1096_RS06315 (D1096_06370)	1411099..1411350	+	252	WP_005598318.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
D1096_RS06320 (D1096_06375)	1411347..1411607	+	261	WP_005605064.1	Txe/YoeB family addiction module toxin	Toxin
D1096_RS06325 (D1096_06380)	1411765..1411932	-	168	WP_009875287.1	Trm112 family protein	-
D1096_RS06330 (D1096_06385)	1411934..1412914	-	981	WP_005608502.1	tetraacyldisaccharide 4'-kinase	-
D1096_RS06335 (D1096_06390)	1413008..1414267	-	1260	WP_005598324.1	ATP-dependent protease ATP-binding subunit ClpX	-
D1096_RS06340 (D1096_06395)	1414267..1414857	-	591	WP_005598326.1	ATP-dependent Clp endopeptidase proteolytic subunit ClpP	-
D1096_RS06345 (D1096_06400)	1414981..1415373	+	393	WP_005618838.1	SufE family protein	-
D1096_RS06360 (D1096_06415)	1415732..1416505	-	774	WP_012263166.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10423.99 Da
• Isoelectric Point: 8.0109

>T110457 WP_005605064.1 NZ_CP031874:1411347-1411607 [Actinobacillus pleuropneumoniae]
MKLTFSSNAWEDYLYWQKTDKIILKRINSLIKDIQRQPFEGIGKLEPLKFNLSGFWSRRINEEHRLIYSVEDEAILIVAC
RYHYDQ

Nucleotide

• Length: 261 bp

>T110457 NZ_CP031874:1411347-1411607 [Actinobacillus pleuropneumoniae]
ATGAAGCTAACTTTCTCGTCTAATGCGTGGGAAGATTATTTATACTGGCAAAAAACCGATAAAATAATTTTGAAACGTAT
AAACAGCCTGATCAAAGATATTCAACGCCAACCATTCGAAGGCATAGGAAAACTGGAGCCGCTAAAATTCAATCTTTCGG
GATTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACAGCGTGGAAGATGAAGCAATTTTAATAGTAGCGTGT
AGATACCATTATGATCAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9362.66 Da
• Isoelectric Point: 4.5659

>AT110457 WP_005598318.1 NZ_CP031874:1411099-1411350 [Actinobacillus pleuropneumoniae]
MQAITYTEARQNLAGTMSKVAEDFEPILITRSKGGNCVLMSYEQYCSLEETAYLMRSPTNAKRLLDSVAELRQGQGIERE
LIE

Nucleotide

• Length: 252 bp

>AT110457 NZ_CP031874:1411099-1411350 [Actinobacillus pleuropneumoniae]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATCTAGCCGGTACGATGTCAAAAGTAGCAGAAGATTTTGAGCCTAT
TTTAATTACAAGAAGCAAGGGCGGAAATTGCGTACTTATGTCGTATGAGCAGTATTGTTCACTTGAAGAAACCGCCTATT
TGATGCGTTCACCCACCAATGCAAAACGGCTATTGGATAGTGTGGCGGAATTAAGACAGGGACAAGGCATTGAAAGAGAG
CTAATCGAATGA

Structures

Toxin

• AlphaFold DB: B0BQK9

Antitoxin

• AlphaFold DB: B0BQK8