Detailed information of TA system TA110456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 714651..715301
• Replicon: chromosome
• Accession: NZ_CP031874
• Organism: Actinobacillus pleuropneumoniae strain S1421

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B0BNU3
• Locus tag: D1096_RS03175
• Protein ID: WP_005614926.1
• Coordinates: 714651..715040 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BNU4
• Locus tag: D1096_RS03180
• Protein ID: WP_005604117.1
• Coordinates: 715041..715301 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1096_RS03165 (D1096_03175)	709891..713166	-	3276	WP_012262906.1	helicase-related protein	-
D1096_RS03170 (D1096_03180)	713292..714515	-	1224	WP_012262907.1	ATP-binding protein	-
D1096_RS03175 (D1096_03185)	714651..715040	-	390	WP_005614926.1	type II toxin-antitoxin system VapC family toxin	Toxin
D1096_RS03180 (D1096_03190)	715041..715301	-	261	WP_005604117.1	hypothetical protein	Antitoxin
D1096_RS03185 (D1096_03195)	715647..716483	+	837	WP_012262908.1	DUF6216 family protein	-
D1096_RS03190 (D1096_03200)	716515..718536	-	2022	WP_012262909.1	excinuclease ABC subunit UvrB	-
D1096_RS03195 (D1096_03210)	718713..719525	+	813	WP_005607533.1	iron uptake system protein EfeO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14837.17 Da
• Isoelectric Point: 5.1295

>T110456 WP_005614926.1 NZ_CP031874:c715040-714651 [Actinobacillus pleuropneumoniae]
MYMLDTNTVSYFFRQDPTVVKKLQQLNPELICISSVTAAELFYGVKKRNNQKLTAFLNTFLSAITVMDWDYQVAEVYGQL
RAEMEREGKIMGVQDQMIGAHALATECVLVSSDKAFQFIPNLVLENWWK

Nucleotide

• Length: 390 bp

>T110456 NZ_CP031874:c715040-714651 [Actinobacillus pleuropneumoniae]
ATGTATATGCTAGATACTAATACGGTGAGTTATTTCTTTAGGCAAGATCCTACTGTGGTTAAGAAACTACAACAACTTAA
TCCGGAATTGATTTGTATTTCATCGGTCACGGCTGCAGAGTTGTTTTATGGCGTAAAAAAACGTAATAATCAGAAACTTA
CTGCATTTTTAAATACTTTTTTATCTGCGATTACGGTGATGGATTGGGATTATCAAGTGGCGGAAGTTTATGGTCAGCTG
CGAGCAGAGATGGAACGAGAAGGCAAAATCATGGGCGTTCAAGATCAAATGATTGGAGCTCATGCCCTAGCAACAGAATG
TGTTCTTGTTTCCAGCGATAAAGCCTTTCAATTTATCCCTAATCTCGTATTAGAAAATTGGTGGAAGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10289.63 Da
• Isoelectric Point: 5.1867

>AT110456 WP_005604117.1 NZ_CP031874:c715301-715041 [Actinobacillus pleuropneumoniae]
MEKVARLFRNGRNQAVRLPVEFEFNAKQVYVFKEANGDITLSTRSRNQQNWQKVFELLPMLECDEQFLSEQERKQEMATR
DPFEGF

Nucleotide

• Length: 261 bp

>AT110456 NZ_CP031874:c715301-715041 [Actinobacillus pleuropneumoniae]
ATGGAAAAGGTCGCACGTTTATTTAGAAACGGACGTAATCAAGCCGTACGATTACCGGTTGAATTTGAGTTTAATGCGAA
GCAAGTTTATGTGTTTAAAGAAGCAAACGGCGATATTACGCTTTCGACACGTTCTCGCAATCAGCAAAATTGGCAGAAAG
TCTTTGAGTTATTACCAATGCTTGAATGTGATGAACAGTTTTTATCGGAACAAGAACGCAAGCAAGAAATGGCAACTCGA
GATCCGTTCGAGGGCTTTTAA

Structures

Toxin

• AlphaFold DB: A3N029

Antitoxin

• AlphaFold DB: A3N030