Detailed information of TA system TA110454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1761347..1762078
• Replicon: chromosome
• Accession: NZ_CP031873
• Organism: Actinobacillus pleuropneumoniae serovar 4 str. M62

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: B0BRD2
• Locus tag: D1097_RS08225
• Protein ID: WP_005605461.1
• Coordinates: 1761347..1761814 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: B0BRD3
• Locus tag: D1097_RS08230
• Protein ID: WP_005619269.1
• Coordinates: 1761818..1762078 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1097_RS08200 (D1097_08220)	1756702..1757073	-	372	WP_005605451.1	CrcB family protein	-
D1097_RS08205 (D1097_08225)	1757076..1758140	-	1065	WP_043990971.1	MFS transporter	-
D1097_RS08210 (D1097_08230)	1758235..1759344	+	1110	WP_005605455.1	anhydro-N-acetylmuramic acid kinase	-
D1097_RS08215 (D1097_08235)	1759398..1760312	+	915	WP_005605457.1	N-acetylmuramic acid 6-phosphate etherase	-
D1097_RS08220 (D1097_08240)	1760382..1761263	-	882	WP_043990972.1	50S ribosomal protein L11 methyltransferase	-
D1097_RS08225 (D1097_08245)	1761347..1761814	-	468	WP_005605461.1	GNAT family N-acetyltransferase	Toxin
D1097_RS08230 (D1097_08250)	1761818..1762078	-	261	WP_005619269.1	DUF1778 domain-containing protein	Antitoxin
D1097_RS08235 (D1097_08255)	1762199..1763659	-	1461	WP_005605463.1	metalloprotease TldD	-
D1097_RS08240 (D1097_08260)	1763789..1765048	+	1260	WP_005605465.1	tRNA lysidine(34) synthetase TilS	-
D1097_RS08245 (D1097_08265)	1765075..1765365	-	291	WP_005598848.1	DUF5389 family protein	-
D1097_RS08250 (D1097_08270)	1765367..1766260	-	894	WP_005605467.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (40-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17276.25 Da
• Isoelectric Point: 9.5125

>T110454 WP_005605461.1 NZ_CP031873:c1761814-1761347 [Actinobacillus pleuropneumoniae serovar 4 str. M62]
MHAPELLSEQHIVRYFQCGEVVLDQWLQRTALKNQQNNASKTFVVRDENKQVMGFYCLSAGSVSHQFVAGALRRNMPDPI
PVIILGRLAVDCSAQGKQLGVSMLKDAVLKSKTVANQIGVKALLVHALNQQAKQFYLKYGFSCSPIDEMVLMLKL

Nucleotide

• Length: 468 bp

>T110454 NZ_CP031873:c1761814-1761347 [Actinobacillus pleuropneumoniae serovar 4 str. M62]
ATGCACGCACCGGAACTGCTTTCCGAGCAACATATTGTGCGATATTTTCAATGTGGTGAGGTCGTGCTAGACCAATGGCT
ACAACGTACTGCATTGAAAAATCAGCAAAATAATGCCAGTAAAACCTTTGTTGTGCGTGATGAGAATAAACAGGTGATGG
GATTTTATTGTTTATCTGCCGGTTCGGTGAGCCATCAGTTTGTTGCAGGCGCATTAAGACGTAATATGCCCGATCCGATT
CCCGTGATTATTTTAGGTAGATTAGCGGTCGATTGCTCAGCACAGGGTAAGCAGTTAGGCGTATCAATGTTAAAAGATGC
GGTCTTAAAATCGAAGACGGTAGCGAATCAAATCGGGGTAAAAGCATTGCTTGTTCATGCGTTAAATCAGCAAGCAAAAC
AATTTTATTTAAAGTACGGTTTTTCATGTTCACCCATTGATGAGATGGTTTTAATGTTAAAACTTTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9796.14 Da
• Isoelectric Point: 4.4883

>AT110454 WP_005619269.1 NZ_CP031873:c1762078-1761818 [Actinobacillus pleuropneumoniae serovar 4 str. M62]
MRTAPINLRAMPDQRDLIDYAASILGKTRSDFMLETACQAAQNVILDRTVFQLNEQAFEQFNRLLEQPTADNPGLDKLLN
TKAVWE

Nucleotide

• Length: 261 bp

>AT110454 NZ_CP031873:c1762078-1761818 [Actinobacillus pleuropneumoniae serovar 4 str. M62]
ATGAGAACTGCGCCAATTAATTTACGAGCGATGCCTGATCAGCGTGATTTAATTGATTATGCGGCAAGCATTTTAGGTAA
AACACGTTCTGACTTTATGTTAGAAACCGCCTGCCAAGCTGCCCAAAATGTGATTTTAGATCGTACCGTTTTCCAATTAA
ATGAGCAGGCTTTCGAGCAATTTAACCGATTGCTAGAACAGCCAACAGCAGATAATCCGGGGTTAGATAAACTGTTAAAT
ACCAAAGCGGTGTGGGAGTAG

Structures

Toxin

• AlphaFold DB: B0BRD2

Antitoxin

• AlphaFold DB: A3N2I7