Detailed information of TA system TA110452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 1461253..1461761
• Replicon: chromosome
• Accession: NZ_CP031873
• Organism: Actinobacillus pleuropneumoniae serovar 4 str. M62

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B0BQK9
• Locus tag: D1097_RS06730
• Protein ID: WP_005605064.1
• Coordinates: 1461501..1461761 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A3N1S6
• Locus tag: D1097_RS06725
• Protein ID: WP_005605062.1
• Coordinates: 1461253..1461504 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1097_RS06680 (D1097_06700)	1456665..1457213	-	549	WP_005605049.1	type IV pilus biogenesis/stability protein PilW	-
D1097_RS06685 (D1097_06705)	1457267..1458448	-	1182	WP_005605050.1	bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN	-
D1097_RS06715 (D1097_06735)	1459476..1460915	+	1440	WP_005605060.1	glutamate--tRNA ligase	-
D1097_RS06725 (D1097_06745)	1461253..1461504	+	252	WP_005605062.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
D1097_RS06730 (D1097_06750)	1461501..1461761	+	261	WP_005605064.1	Txe/YoeB family addiction module toxin	Toxin
D1097_RS06735 (D1097_06755)	1461920..1462087	-	168	WP_005605066.1	Trm112 family protein	-
D1097_RS06740 (D1097_06760)	1462089..1463069	-	981	WP_005605069.1	tetraacyldisaccharide 4'-kinase	-
D1097_RS06745 (D1097_06765)	1463164..1464423	-	1260	Protein_1317	ATP-dependent protease ATP-binding subunit ClpX	-
D1097_RS06750 (D1097_06770)	1464423..1465013	-	591	WP_005598326.1	ATP-dependent Clp endopeptidase proteolytic subunit ClpP	-
D1097_RS06755 (D1097_06775)	1465135..1465527	+	393	WP_005620423.1	SufE family protein	-
D1097_RS06770 (D1097_06790)	1465886..1466659	-	774	WP_043990950.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10423.99 Da
• Isoelectric Point: 8.0109

>T110452 WP_005605064.1 NZ_CP031873:1461501-1461761 [Actinobacillus pleuropneumoniae serovar 4 str. M62]
MKLTFSSNAWEDYLYWQKTDKIILKRINSLIKDIQRQPFEGIGKLEPLKFNLSGFWSRRINEEHRLIYSVEDEAILIVAC
RYHYDQ

Nucleotide

• Length: 261 bp

>T110452 NZ_CP031873:1461501-1461761 [Actinobacillus pleuropneumoniae serovar 4 str. M62]
ATGAAGCTAACTTTCTCGTCTAATGCGTGGGAAGATTATTTATACTGGCAAAAAACCGATAAAATAATTTTGAAACGTAT
AAACAGTCTGATTAAAGATATTCAACGCCAACCATTTGAAGGCATAGGAAAACTGGAGCCGCTAAAATTCAATCTTTCGG
GATTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACAGCGTGGAAGATGAAGCAATTTTAATAGTAGCGTGT
AGATACCATTATGATCAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9320.62 Da
• Isoelectric Point: 4.5659

>AT110452 WP_005605062.1 NZ_CP031873:1461253-1461504 [Actinobacillus pleuropneumoniae serovar 4 str. M62]
MQAITYTEARQNLAGTMSKVAEDFEPILITRSKGGNCVLMSYEQYSSLEETAYLMRSPANAQRLLDSMAELKQGKGIERE
LIE

Nucleotide

• Length: 252 bp

>AT110452 NZ_CP031873:1461253-1461504 [Actinobacillus pleuropneumoniae serovar 4 str. M62]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATCTAGCCGGTACAATGTCAAAAGTAGCAGAAGATTTTGAGCCTAT
TTTAATTACAAGAAGCAAGGGCGGAAATTGCGTACTTATGTCGTATGAGCAGTATAGTTCACTTGAAGAAACCGCCTATT
TAATGCGTTCCCCGGCTAACGCTCAAAGATTACTAGACAGCATGGCAGAATTGAAGCAAGGGAAAGGCATTGAAAGAGAG
CTAATCGAATGA

Structures

Toxin

• AlphaFold DB: B0BQK9

Antitoxin

• AlphaFold DB: A3N1S6