Detailed information of TA system TA110449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1703252..1703880
• Replicon: chromosome
• Accession: NZ_CP031869
• Organism: Actinobacillus pleuropneumoniae strain WF83

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D1100_RS08060
• Protein ID: WP_005618201.1
• Coordinates: 1703252..1703650 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B3GYV7
• Locus tag: D1100_RS08065
• Protein ID: WP_005613300.1
• Coordinates: 1703650..1703880 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1100_RS08035 (D1100_08085)	1698874..1699395	+	522	WP_005618211.1	ATP-dependent protease subunit HslV	-
D1100_RS08040 (D1100_08090)	1699450..1700772	+	1323	WP_005620148.1	HslU--HslV peptidase ATPase subunit	-
D1100_RS08045 (D1100_08095)	1700845..1701624	+	780	WP_005602546.1	hypothetical protein	-
D1100_RS08050 (D1100_08100)	1701804..1702454	-	651	WP_005618207.1	DUF2202 domain-containing protein	-
D1100_RS08055 (D1100_08105)	1702700..1703116	+	417	WP_005618204.1	DUF417 family protein	-
D1100_RS08060 (D1100_08110)	1703252..1703650	-	399	WP_005618201.1	tRNA(fMet)-specific endonuclease VapC	Toxin
D1100_RS08065 (D1100_08115)	1703650..1703880	-	231	WP_005613300.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D1100_RS08070 (D1100_08120)	1704401..1706032	-	1632	WP_005618198.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1678609..1718562	39953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (6-53)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15124.41 Da
• Isoelectric Point: 6.9683

>T110449 WP_005618201.1 NZ_CP031869:c1703650-1703252 [Actinobacillus pleuropneumoniae]
MLTYMLDTNIAIYVIKRRPIEVLDKFNLNSTRLCVSSITAAELYYDAEKSQFPERNMAVIEDFLSRLTILDYTHKAATHF
GNIKAHLSKCGKILGENDIHIAAHARSEGLVLVTNNLREFERVEGLRLDNWV

Nucleotide

• Length: 399 bp

>T110449 NZ_CP031869:c1703650-1703252 [Actinobacillus pleuropneumoniae]
ATGCTGACTTATATGCTTGATACTAATATTGCGATTTATGTGATTAAACGCAGACCTATTGAAGTGCTGGATAAGTTTAA
TTTAAATTCTACTCGTCTTTGTGTAAGTAGTATCACTGCAGCGGAGCTGTATTATGATGCAGAGAAAAGCCAGTTTCCGG
AACGAAATATGGCAGTAATTGAGGATTTTCTTTCTCGTTTAACAATTCTTGATTATACGCATAAGGCGGCTACTCACTTT
GGCAATATCAAAGCACATTTATCTAAGTGCGGTAAGATTCTCGGCGAGAACGATATTCATATTGCCGCGCATGCAAGAAG
TGAAGGACTCGTATTAGTGACCAATAACTTGCGTGAATTTGAACGTGTGGAAGGATTGCGATTAGATAATTGGGTATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8625.71 Da
• Isoelectric Point: 4.5358

>AT110449 WP_005613300.1 NZ_CP031869:c1703880-1703650 [Actinobacillus pleuropneumoniae]
MEASVFMTNRSQAVRLPAEVRFASDVKKLSVRVVGNERILAPINQSWDSFFLSSATVSDDFMTEREISTQAEREEL

Nucleotide

• Length: 231 bp

>AT110449 NZ_CP031869:c1703880-1703650 [Actinobacillus pleuropneumoniae]
ATGGAAGCATCAGTATTTATGACAAATCGTAGTCAAGCGGTACGATTGCCGGCAGAAGTACGTTTTGCAAGTGATGTAAA
AAAACTTTCTGTAAGAGTAGTTGGTAATGAGCGTATTTTGGCACCGATTAACCAAAGTTGGGATAGCTTCTTTCTAAGTT
CGGCAACGGTAAGCGATGATTTTATGACAGAGCGTGAAATAAGCACTCAAGCTGAACGTGAGGAACTGTAA

Structures

Antitoxin

• AlphaFold DB: A3N328