Detailed information of TA system TA110448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 1604467..1605182
• Replicon: chromosome
• Accession: NZ_CP031869
• Organism: Actinobacillus pleuropneumoniae strain WF83

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D1100_RS07470
• Protein ID: WP_005599445.1
• Coordinates: 1604817..1605182 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BT01
• Locus tag: D1100_RS07465
• Protein ID: WP_005599447.1
• Coordinates: 1604467..1604754 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1100_RS07440 (D1100_07485)	1600070..1600663	-	594	WP_005599453.1	protein-methionine-sulfoxide reductase heme-binding subunit MsrQ	-
D1100_RS07445 (D1100_07490)	1600663..1601619	-	957	WP_005619040.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
D1100_RS07450 (D1100_07495)	1601708..1602289	+	582	WP_005599450.1	sigma-70 family RNA polymerase sigma factor	-
D1100_RS07455 (D1100_07500)	1602292..1602480	+	189	WP_005618305.1	hypothetical protein	-
D1100_RS07460 (D1100_07505)	1602546..1604261	-	1716	WP_005618303.1	proline--tRNA ligase	-
D1100_RS07465 (D1100_07510)	1604467..1604754	+	288	WP_005599447.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
D1100_RS07470 (D1100_07515)	1604817..1605182	+	366	WP_005599445.1	type II toxin-antitoxin system VapC family toxin	Toxin
D1100_RS07475 (D1100_07520)	1605306..1607387	+	2082	WP_237601098.1	ATP-dependent DNA helicase RecG	-
D1100_RS07480 (D1100_07525)	1607514..1607795	+	282	WP_005599441.1	DNA-directed RNA polymerase subunit omega	-
D1100_RS07485 (D1100_07530)	1607891..1609597	+	1707	WP_237593915.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13974.06 Da
• Isoelectric Point: 9.0549

>T110448 WP_005599445.1 NZ_CP031869:1604817-1605182 [Actinobacillus pleuropneumoniae]
MDTNVRQWLATIKPSQTNISCITLSEIKTGILLKARKDPIQAERLNHWFTHNVLSVYQAKSFSINNEIALLASEYHIPNK
MDLNDAYIAATAKYHNLVLVTRNLKDFNRCDIRLFNPFEPN

Nucleotide

• Length: 366 bp

>T110448 NZ_CP031869:1604817-1605182 [Actinobacillus pleuropneumoniae]
ATGGATACCAATGTACGACAATGGTTAGCAACGATTAAACCGAGTCAGACGAATATTAGCTGTATCACGCTTTCCGAAAT
AAAAACGGGGATTTTACTCAAAGCTCGTAAAGATCCTATTCAAGCCGAGCGGTTAAATCATTGGTTTACCCATAATGTAT
TAAGCGTTTATCAAGCTAAATCCTTTTCTATAAATAATGAAATTGCGTTACTGGCTTCTGAATACCACATTCCAAATAAA
ATGGATTTAAACGATGCTTATATTGCCGCAACCGCTAAATATCATAATTTAGTGCTTGTTACTCGTAATTTGAAAGACTT
CAACCGTTGCGATATACGTTTATTTAATCCTTTCGAACCGAATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11040.29 Da
• Isoelectric Point: 4.5578

>AT110448 WP_005599447.1 NZ_CP031869:1604467-1604754 [Actinobacillus pleuropneumoniae]
MPITITSREFNQHASKVLKMAEKEPVFITKWGKIVSVVSNYVDYKQHTTVEPSFEELFASSTDESVSDEFWQDFEQTLTK
IRKSNKSEQIDFGDE

Nucleotide

• Length: 288 bp

>AT110448 NZ_CP031869:1604467-1604754 [Actinobacillus pleuropneumoniae]
ATGCCAATTACTATTACCTCTCGAGAATTTAATCAACACGCCAGTAAAGTGCTGAAAATGGCAGAAAAAGAACCGGTTTT
TATCACTAAATGGGGAAAAATTGTCAGCGTTGTCTCCAATTATGTTGATTACAAACAGCACACAACGGTTGAGCCTAGCT
TTGAAGAACTATTTGCCTCATCTACGGATGAATCGGTTAGCGATGAGTTTTGGCAAGATTTTGAACAGACATTAACAAAA
ATTCGCAAAAGTAATAAATCCGAACAAATAGATTTTGGAGATGAATAA

Structures

Antitoxin

• AlphaFold DB: A3N3C5