Detailed information of TA system TA110447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxTA/Tad-couple_hipB
• Location: 1570423..1571068
• Replicon: chromosome
• Accession: NZ_CP031869
• Organism: Actinobacillus pleuropneumoniae strain WF83

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: B0BQU7
• Locus tag: D1100_RS07270
• Protein ID: WP_005619674.1
• Coordinates: 1570697..1571068 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: D1100_RS07265
• Protein ID: WP_005598477.1
• Coordinates: 1570423..1570716 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1100_RS07235 (D1100_07285)	1565755..1566438	-	684	WP_005605188.1	arginine ABC transporter permease ArtM	-
D1100_RS07240 (D1100_07290)	1566438..1567109	-	672	WP_005617821.1	arginine ABC transporter permease ArtQ	-
D1100_RS07245 (D1100_07295)	1567115..1567834	-	720	WP_012478527.1	transporter substrate-binding domain-containing protein	-
D1100_RS07250 (D1100_07300)	1567854..1568588	-	735	WP_005598473.1	arginine ABC transporter ATP-binding protein ArtP	-
D1100_RS07255 (D1100_07305)	1568813..1569310	-	498	WP_005617825.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
D1100_RS07260 (D1100_07310)	1569383..1570345	+	963	WP_012478528.1	calcium/sodium antiporter	-
D1100_RS07265 (D1100_07315)	1570423..1570716	-	294	WP_005598477.1	helix-turn-helix domain-containing protein	Antitoxin
D1100_RS07270 (D1100_07320)	1570697..1571068	-	372	WP_005619674.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D1100_RS07275 (D1100_07325)	1571262..1573013	+	1752	WP_237601096.1	protein-disulfide reductase DsbD	-
D1100_RS07280 (D1100_07330)	1573071..1573640	+	570	WP_005608594.1	elongation factor P hydroxylase	-
D1100_RS07285 (D1100_07335)	1573684..1574538	-	855	WP_005615766.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (32-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-85)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 15066.57 Da
• Isoelectric Point: 10.4054

>T110447 WP_005619674.1 NZ_CP031869:c1571068-1570697 [Actinobacillus pleuropneumoniae]
MYEIVFYRDKRGREPVKEFLLRLLKERQEGSRERLHKISHHLSILHLHGTRAGENYIKHLEDRIWQLRPVGDCLLFASII
RGKFVLLHYFAKQNYRIPKREIERAKVRLADLQERIKDEPYWF

Nucleotide

• Length: 372 bp

>T110447 NZ_CP031869:c1571068-1570697 [Actinobacillus pleuropneumoniae]
ATGTATGAAATAGTCTTTTATCGAGATAAGCGGGGGAGAGAACCCGTAAAAGAATTTCTGTTAAGGCTACTAAAAGAACG
GCAGGAAGGCAGTCGAGAACGATTACACAAAATCAGCCATCATTTGAGTATTTTACATTTGCACGGTACTCGAGCCGGTG
AGAATTATATTAAGCATTTGGAAGATCGTATTTGGCAGTTACGTCCGGTGGGCGATTGCCTGTTATTTGCAAGTATTATT
CGGGGAAAATTTGTGTTGTTGCATTATTTTGCCAAACAGAATTATCGAATTCCGAAACGAGAAATAGAACGTGCAAAAGT
TCGGTTGGCGGATTTACAGGAGAGAATAAAAGATGAGCCCTATTGGTTCTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10935.81 Da
• Isoelectric Point: 10.3120

>AT110447 WP_005598477.1 NZ_CP031869:c1570716-1570423 [Actinobacillus pleuropneumoniae]
MSPIGSSWTSFEQEIFDESEIAETQFRIRLILEIIETRQQLGISQRKLEKLSGVKQSMIARVEKGSVNPSLATVLKLLVP
LGKTLQIVPLDKNKTVR

Nucleotide

• Length: 294 bp

>AT110447 NZ_CP031869:c1570716-1570423 [Actinobacillus pleuropneumoniae]
ATGAGCCCTATTGGTTCTAGTTGGACATCGTTTGAACAAGAAATTTTTGACGAGTCGGAAATAGCGGAAACTCAGTTTCG
CATACGACTTATTTTAGAAATCATTGAAACCCGTCAGCAGCTCGGTATCTCTCAACGAAAATTGGAGAAATTAAGCGGCG
TAAAACAATCTATGATTGCTCGGGTAGAAAAAGGCTCGGTTAATCCGTCGTTGGCGACGGTATTAAAGTTGCTTGTGCCG
TTGGGGAAAACTTTACAAATCGTACCGTTAGATAAAAACAAAACGGTACGTTAA

Structures

Toxin

• AlphaFold DB: A3N206